Metabolomics,Multiomics

Dataset Information

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Metabolomics Analysis Reveals Tissue-Specific Metabolite Compositions in Leaf Blade and Traps of Carnivorous Nepenthes Plants


ABSTRACT: Nepenthes is a genus of carnivorous plants that evolved a pitfall trap, the pitcher, to catch and digest insect prey to obtain additional nutrients. Each pitcher is part of the whole leaf, together with a leaf blade. These two completely different parts of the same organ were studied separately in a non-targeted metabolomics approach in Nepenthes x ventrata, a robust natural hybrid. The first aim was the analysis and profiling of small (50-1000 m/z) polar and non-polar molecules to find a characteristic metabolite pattern for the particular tissues. Second, the impact of insect feeding on the metabolome of the pitcher and leaf blade was studied. Using UPLC-ESI-qTOF and cheminformatics, about 2000 features (MS/MS events) were detected in the two tissues. They showed a huge chemical diversity, harboring classes of chemical substances that significantly discriminate these tissues. Among the common constituents of N. x ventrata are phenolics, flavonoids and naphthoquinones, namely plumbagin, a characteristic compound for carnivorous Nepenthales, and many yet-unknown compounds. Upon insect feeding, only in pitchers in the polar compounds fraction, small but significant differences could be detected. By further integrating information with cheminformatics approaches, we provide and discuss evidence that the metabolite composition of the tissues can point to their function.

OTHER RELATED OMICS DATASETS IN: PXD009709PXD018322PXD006154

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase, Liquid Chromatography MS - Positive (LC-MS (Positive))

SUBMITTER: Carlos RodrÃguez-López 

PROVIDER: MTBLS1783 | MetaboLights | 2020-07-10

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS1783 Other
FILES Other
a_MTBLS1783_lipids_LC-MS.txt Txt
a_MTBLS1783_polar_LC-MS.txt Txt
i_Investigation.txt Txt
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Publications

Metabolomics Analysis Reveals Tissue-Specific Metabolite Compositions in Leaf Blade and Traps of Carnivorous <i>Nepenthes</i> Plants.

Dávila-Lara Alberto A   Rodríguez-López Carlos E CE   O'Connor Sarah E SE   Mithöfer Axel A  

International journal of molecular sciences 20200619 12


<i>Nepenthes</i> is a genus of carnivorous plants that evolved a pitfall trap, the pitcher, to catch and digest insect prey to obtain additional nutrients. Each pitcher is part of the whole leaf, together with a leaf blade. These two completely different parts of the same organ were studied separately in a non-targeted metabolomics approach in <i>Nepenthes x ventrata,</i> a robust natural hybrid. The first aim was the analysis and profiling of small (50-1000 <i>m</i>/<i>z</i>) polar and non-pola  ...[more]

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