Proteomics

Dataset Information

0

Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine


ABSTRACT: Extracellular vesicles (EVs) have a great potential in clinical applications, however their isolation from different body fluids and their characterisation are not yet optimal or standardised. Here we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is a fast method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with the applicabilities and limitations of current methods. We demonstrate that AF4/UV-MALS is a straightforward characterisation method for urinary EVs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Kurt Boonen  

LAB HEAD: Inge Mertens

PROVIDER: PXD009709 | Pride | 2018-07-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ureum_pellet_hydro_4.raw Raw
ureum_pellet_hydro_456.msf Msf
ureum_pellet_hydro_5.raw Raw
ureum_pellet_hydro_6.raw Raw
Items per page:
1 - 4 of 4
altmetric image

Publications

Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine.

Oeyen Eline E   Van Mol Kurt K   Baggerman Geert G   Willems Hanny H   Boonen Kurt K   Rolfo Christian C   Rolfo Christian C   Pauwels Patrick P   Jacobs An A   Schildermans Karin K   Cho William C WC   Mertens Inge I  

Journal of extracellular vesicles 20180703 1


Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is an efficient method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractio  ...[more]

Similar Datasets

2018-07-23 | PXD007646 | Pride
2023-08-30 | PXD027925 | Pride
2022-02-16 | PXD028712 | Pride
2024-04-30 | PXD049437 | Pride
2024-07-20 | PXD053406 | Pride
2020-05-27 | PXD016087 | Pride
2019-11-25 | PXD013872 | Pride
2021-09-10 | PXD023595 | Pride
2020-01-29 | PXD016322 | Pride
2009-01-02 | E-GEOD-8882 | biostudies-arrayexpress