Dataset Information

Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics

ABSTRACT:

Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled ca. 60 days post-anthesis over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics. To complement the data, a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids. Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola, while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties. Finally, proteomics on Morellona and Crognola showed variations in proteins involved in stress response, primary metabolism and cell wall expansion. To the best of our knowledge, this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes, proteins and metabolites.

INSTRUMENT(S): UHPLC-DAD-HR-MS/MS

SUBMITTER: Sophie CHARTON

PROVIDER: MTBLS1803 | MetaboLights | 2021-10-29

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS1803	Other
	FILES	Other
	a_MTBLS1803_LC-MC_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS1803_LC-MC_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other

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Publications

Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics.

Berni Roberto R Charton Sophie S Planchon Sébastien S Legay Sylvain S Romi Marco M Cantini Claudio C Cai Giampiero G Hausman Jean-Francois JF Renaut Jenny J Guerriero Gea G

Horticulture research 20210101 1

Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled ca. 60 days post-anthesis over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with me ...[more]

PMID: 33384418

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