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UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications

ABSTRACT:

Real-time breath analysis using secondary electrospray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to access the metabolic state of a person. However, it lacks the ability to unequivocally assign mass spectral features to compounds due to the absence of chromatographic separation. This can be overcomed by using exhaled breath condensate and conventional liquid chromatography-mass spectrometry (LC-MS) systems. In this study, to the best of our knowledge, we confirm for the first time the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr) previously reported to be involved in response to and side effects from antiseizure medications in exhaled breath condensate and by extension in exhaled human breath.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Kapil Dev Singh

PROVIDER: MTBLS6760 | MetaboLights | 2023-01-26

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS6760	Other
	FILES	Other
	a_MTBLS6760_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt

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Publications

UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications.

Awchi Mo M Sinues Pablo P Datta Alexandre N AN García-Gómez Diego D Singh Kapil Dev KD

Journal of proteome research 20230222 3

PMID: 36812155

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