Comparative characterization of the infant gut microbiome and their maternal lineage by a multi-omics approach

ABSTRACT:

The human gut microbiome establishes and matures during infancy, and dysregulation at this stage may lead to pathologies later in life. We conducted a multi-omics study comprising three generations of family members to investigate the early development of the gut microbiota. Fecal samples from 200 individuals, including infants (0-12 months old; 55% females, 45% males) and their respective mothers and grandmothers, were analyzed using two independent metabolomics platforms and metagenomics. For metabolomics, gas chromatography and capillary electrophoresis – using multisegment injection – coupled to mass spectrometry were applied. For metagenomics, both 16S rRNA gene and shotgun sequencing were performed. Here we show that infants greatly vary from their elders in fecal microbiota populations, function, and metabolome. Infants have a less diverse microbiota than adults and present differences in several metabolite classes, such as short- and branched-chain fatty acids, which are associated with shifts in bacterial populations. These findings provide innovative biochemical insights into the shaping of the gut microbiome within the same generational line that could be beneficial in improving childhood health outcomes.

INSTRUMENT(S): Capillary electrophoresis MS - positive, Capillary electrophoresis MS - negative, Gas Chromatography MS - positive

SUBMITTER: Tomas Clive Barker Tejeda 

PROVIDER: MTBLS7670 | MetaboLights | 2024-03-07

REPOSITORIES: MetaboLights