Ontology highlight
ABSTRACT: Divergence in the non-targeted metabolite profilings of bovine mammary epithelial cells (BMECs) were systematically captured with regard to 10 individual essential amino acid (EAA) availability. BMECs cultured in 6-well plates were first serum-starved overnight and subsequently assigned to 1 of 12 treatment media (n = 6). DMEM-F12 medium is the positive control treatment (POS), while DMEM-F12 medium devoid of all EAA served as the negative control treatment (NEG). A total of 10 treatments were NEG individually supplemented with arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan or valine (Sigma-Aldrich, MO, USA). Individual EAA were supplemented to achieve concentrations equal to those of POS. After 6-h treatment, all cell samples were taken at the same time, frozen in liquid nitrogen and stored at -80 °C until required. Untargeted metabolomics is reported in the current study MTBLS7789. Targeted metabolomics is reported in MTBLS3956.
INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic
SUBMITTER: Lianbin Xu
PROVIDER: MTBLS7789 | MetaboLights | 2024-03-04
REPOSITORIES: MetaboLights
Action | DRS | |||
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MTBLS7789 | Other | |||
FILES | Other | |||
a_MTBLS7789_LC-MS_alternating_hilic_metabolite_profiling.txt | Txt | |||
i_Investigation.txt | Txt | |||
m_MTBLS7789_LC-MS_alternating_hilic_metabolite_profiling_v2_maf.tsv | Tabular |
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