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Omics score: 1062

Cold Storage of Rat Hepatocyte Spheroids

ABSTRACT: The purpose of this study was to optimize conditions for cold storage of rat hepatocyte spheroids without freezing. Rat hepatocytes were isolated by a two-step perfusion method; hepatocyte spheroids were formed during 48 h of rocked culture in serum-free medium (SFM). Spheroids were then maintained in rocked culture at 37°C (control condition) or cold stored at 4°C for 24 or 48 h in six different cold storage solutions: SFM alone; SFM + 1 mM deferoxamine (Def); SFM + 1 ?M cyclosporin A (CsA); SFM + 1 mM Def + 1 ?M CsA, University of Wisconsin (UW) solution alone, UW + 1 mM Def.

INSTRUMENT(S): Triple quadrupole

ORGANISM(S): Rat Rattus Norvegicus

TISSUE(S): Liver

SUBMITTER: Scott Nyberg

PROVIDER: ST000167 | MetabolomicsWorkbench | Thu May 14 00:00:00 BST 2015

REPOSITORIES: MetabolomicsWorkbench

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