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T cell metabolism during graft-versus-host disease (CAB 307)-PART I

ABSTRACT: T cells were injected into mice to model graft-versus-host disease. On day 7 after bone marrow transplantation (BMT), cells were recovered and CD8 T cells were purified to > 90% purity over magnetic columns. Naïve T cells were used as control. Cells were then plated at 5x10^6 cells/ml onto plates coated with anti-CD3/CD28 antibodies in the presence of 300µM 13C-palmitate conjugated to BSA (in PBS). Cells were incubated at 37oC for 60min, after which time they were removed from the plates, counted, and an equal number (2.5x10^6) were placed into a 1.7 ml micro-centrifuge tubes and spun down. Cells were washed once with ammonium chloride, residual volume was removed by a second brief spin, cell pellets were flash frozen in liquid nitrogen and stored at -80oC until analysis. Several mice lines were used in this study.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): T-cells

SUBMITTER: Maureen Kachman

PROVIDER: ST000178 | MetabolomicsWorkbench | Sat Jun 06 00:00:00 BST 2015

REPOSITORIES: MetabolomicsWorkbench

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