Metabolomics

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Metabolic profiling of identified single cells in Xenopus laevis embryos


ABSTRACT: Single D11 cells were identified in 16-cell embryos of Xenopus laevis. Metabolites were extracted, and the extracts were analyzed using a custom-built capillary electrophoresis electrospray ionization platform coupled to a quadrupole time-of-flight mass spectrometer. The resulting metadata was analyzed by Trace, a custom-design software, designed to extract molecular feautres from trace-sensitive metabolomics experiments. The results were validated against molecular features that were extracted by manual curation of the same raw mass spectrometer files.

ORGANISM(S): Frog Xenopus Laevis

TISSUE(S): Embryonic Cells

SUBMITTER: Peter Nemes  

PROVIDER: ST001028 | MetabolomicsWorkbench | Wed Jul 25 00:00:00 BST 2018

REPOSITORIES: MetabolomicsWorkbench

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