Dataset Information

Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa (part-I)

ABSTRACT: Lipidomics is a promising tool to determine biomarkers and elucidate mechanisms associated with anthropogenic-induced stress in wildlife. Therefore, we examine the application of lipidomics for in situ studies on Mozambique tilapia (Oreochromis mossambicus) in Loskop Dam, South Africa. Mortality events of aquatic life associated with an environmentally-derived inflammatory disease, pansteatitis, have occurred in this area. The lipidome of adipose tissue (n = 31) and plasma (n = 51) from tilapia collected from at Loskop Dam were characterized using state of the art liquid chromatography coupled to high-resolution tandem mass spectrometry. Lipid profiles reflected pansteatitis severity and were significantly different between diseased and healthy individuals. Over 13 classes of lipids associated with inflammation, cell death, and/or oxidative damage were upregulated in pansteatitis-affected adipose tissue, including ether-lipids, short-chained triglyceride oxidation products, sphingolipids, and acylcarnitines. Ceramides showed a 1000-fold increase in the most affected adipose tissues, illustrating its potential as sensitive and novel indicators of disease severity. In plasma, triglycerides were found to be downregulated in pansteatitis-affected tilapia. As comprehensive coverage of the lipidome aids in the elucidation of possible disease mechanisms, application of lipidomics could be applied to the understanding of other environmentally-derived inflammatory conditions, such as those caused by obesogens.

ORGANISM(S): Fish Oreochromis Mossambicus

TISSUE(S): Blood

DISEASE(S): Inflammation

SUBMITTER: Jeremy Koelmel

PROVIDER: ST001052 | MetabolomicsWorkbench | Thu Sep 13 00:00:00 BST 2018

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	mwtab	Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Rationale: Dieldrin is a legacy organochlorine pesticide that is persistent in the environment, despite being discontinued from use in North America since the 1970s. Some epidemiological studies suggest that exposure to dieldrin is associated with increased risks of neurodegenerative disease and breast cancer by inducing inflammatory responses in tissues as well as oxidative stress. However, the direct effects of organochlorine pesticides on the heart have not been addressed adequately to date. This is a significant knowledge gap because these chemicals are detectable in human serum and are environmentally persistent, thus individuals may show latent adverse effects due to chronic, low dose exposure over time. Objective: To determine whether low level exposure to dieldrin at an environmentally relevant dose results in aberrant molecular signaling in the vertebrate heart. Methods and Results: Using transcriptomic profiling and immunoblotting, we determined the global gene and targeted protein expression response to dieldrin treatment, and show that dieldrin effects gene regulator networks in the heart that are associated with protein degradation. Our results show that genes related to the development of cardiovascular disease, specifically cardiac arrest and ventricular fibrillation, are affected by environmentally relevant levels of dieldrin. We find that genes regulating inflammatory responses, a significant risk factor for cardiovascular disease, are upregulated by dieldrin treatments. Transcripts related to lysosomal function, also important to heart function, are significantly downregulated. To verify these findings, proteins in these pathways were examined with immunoblotting, and our data suggest that dieldrin constitutively activates Akt/mTOR signalling and downregulates lysosomal genes, which is hypothesized to be mediated through Transcription Factor EB (TFEB), the master regulator of lysosome function. Conclusions: This study is one of few to report that dieldrin alters molecular signaling cascades in the cardiovascular system and proposes a novel mechanism for pesticide-induced cardiotoxicity.

Dataset Information

Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa (part-I)

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets