Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts
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ABSTRACT: Human skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility.
ORGANISM(S): Human Homo Sapiens
TISSUE(S): Fibroblast Cells
SUBMITTER: Jordan Wilkins
PROVIDER: ST001148 | MetabolomicsWorkbench | Wed Feb 27 00:00:00 GMT 2019
REPOSITORIES: MetabolomicsWorkbench
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