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Design of Experiments for Maximizing T cell endpoints

ABSTRACT: Purified T cells from a single healthy donor were expanded in vitro with either magnetic beads or degradable micro-scaffold (DMS) particles. Magnetic bead cultures were expanded according to manufacturer’s instructions, while DMS cultures were expanded with varying DMS particle concentration, IL2 concentration, and antigen surface density on the particles, according to a design of experiments. Media of each culture of was sampled repeatedly over the course of a 14 day period. Quantities and characteristics of activated T cells were assessed at the end of the culture period, and media was analyzed by 1H-NMR. Analysis of spectra resulted in a set of 20 features that was used in predictive modeling of T cell endpoints, along with culture parameters described above and cytokine data. A second validation experiment was performed with different values for culture parameters, using the same culture, sampling, and NMR analysis methods.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): T-cells

SUBMITTER: Max Colonna

PROVIDER: ST001476 | MetabolomicsWorkbench | Wed Aug 12 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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