Metabolomics dataset of optogenetic axon regenerative mouse model post optic nerve crush
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ABSTRACT: This metabolite dataset was collected from bacterial channel rhodopsin expressing transgenic mouse models subjected to optic nerve crush (ONC) with subsequent stimulation with light (promoting regeneration) or non-stimulation (lacking axon regeneration). ONC retains retinal ganglion cells within the retina, while degenerating axons. In transgenic bacterial channel rhodopsin expressing cells, light stimulation promotes regeneration. Genetically matched wild-type uninjured optic nerves, or control transgenic mice, were also analyzed. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 Methanol and H2O and second extraction using 8:1:1 of Acetonitrile:Acetone:Methanol). Untargeted liquid chromatography-mass spectrometry (LC-MS/MS) profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound DiscovererTM software. Statistical analysis was performed using MetaboAnalyst 5.0.
ORGANISM(S): Mouse Mus Musculus
TISSUE(S): Eye Tissue
DISEASE(S): Eye Disease
SUBMITTER: Sanjoy K. Bhattacharya
PROVIDER: ST002111 | MetabolomicsWorkbench | Tue Mar 08 00:00:00 GMT 2022
REPOSITORIES: MetabolomicsWorkbench
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