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Lipidomics analysis on Arabidopsis autophagy mutants

ABSTRACT: Autophagy is an essential cellular process in eukaryotes that degrades and recycles macromolecules and organelles. Defects in autophagy is known to affect metabolism, including the lipidome. Genetic approaches have identified a series of AuTophaGy-related (ATG) genes in Arabidopsis. In this study we used WT (ecotype Col-0) and two Arabidopsis autophagy-defective mutants, atg7 and atg9 to perform a multi-omics study on the effect of nitrogen starvation treatment, which induces autophagy. Specifically, we have quantified ~100 lipids from leaf and root tissues of WT, atg7 and atg9 mutant plants, under either autophagy-inducing conditions (-N) or normal nitrogen conditions (+N). The lipid species we quantified include: DGDG, MGDG, LPC, LPE, PE, LPG, PC, PA, PG, PI, and PS. Our study sheds lights on the understanding of the relationships between autophagy and metabolism, especially lipid metabolism.

ORGANISM(S): Arabidopsis Thaliana

TISSUE(S): Plant

SUBMITTER: Geng Ding

PROVIDER: ST002252 | MetabolomicsWorkbench | Mon Feb 21 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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