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Bloody Mary (BM21)- Serial mixtures of vegetable juice /water and human plasma.

ABSTRACT: Metabolomics holds the promise to measure and quantify small molecules comprehensively in biological systems, and LC-MS (liquid chromatography coupled mass spectrometry) has become the leading technology in the field. Significant challenges still exist in the computational processing of data from LC-MS metabolomic experiments into metabolite features, including provenance and reproducibility of the current software tools. We present here, an experiment designed as serial mixtures of vegetable juice/Water and human plasma at varying ratios, nicknamed “Bloody Mary 21(BM21) to test semi-quantification at –omics scale. A subset of features are expected to have their peak areas correlated with the mixing ratio. This dataset provides an opportunity to be used as benchmark to assess the performance in quantification of processing softwares. Overall, the BM21 experiment included a serial mixture of human plasma (Qstd) and vegetable juice (or water), at the ratio of 1024:1, 256:1, 64:1, 16:1, 4:1, 1:1, 1:4, 1:16, 1:64, 1:256 and 1:1024. Along with the 11 serial mixture samples, 100% vegetable juice and 100% plasma were also included. All samples were analyzed in triplicates.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Blood

SUBMITTER: Amnah Siddiqa

PROVIDER: ST002454 | MetabolomicsWorkbench | Mon Jun 27 00:00:00 BST 2022

REPOSITORIES: MetabolomicsWorkbench

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Project description:BACKGROUND: Evidence on the relationship between food processing and health outcomes has been increasing steadily. Under this context, ultra-processing food, such as sugar-sweetened fruity beverages (SS-FB), is associated with a higher risk of metabolic syndrome. However, whether drinking fruit juice not from concentrate (NFC-FJ), which belongs to minimally processed food, causes poor health or not is controversial. The major goal was to determine the impact of fruit drinks with different processing degrees on health via the interaction of gut microbiota and metabolomics.RESULTS: Significant different trends in metabolic syndrome were found between NFC-FJ and SS-FB in a 4-week experiment. NFC-FJ increased alpha diversity and decreased F/B ratio compared to SS-FB. The modulated bacterial taxa by NFC-FJ increased lipid metabolism as well as decreased insulin resistance and inhibited the dimethyl-L-arginine (ADMA) formation from S-adenosyl-L-methionine (SAM), contributing to a lower risk of metabolic syndrome in rats. However, SS-FB led to a higher risk of metabolic syndrome in rats with increased food intake and weight change. Additionally, SS-FB with the fructose-to-glucose ratio of 1.5 corresponded to an increase in kidney mass and serum C17-sphinganine level, boosting risks for kidney disease. More than 10 times higher flavonoid content in NFC-FJ than SS-FB was caused by processing degrees, which might be partly responsible for the alteration of gut microbiota.CONCLUSIONS: Our results revealed that rich flavonoids in minimally processed fruit juice decreased the risk of metabolic syndrome in rats through the pathway of insulin resistance via gut microbiota alteration, while ultra-processed fruit beverages enhanced the risk of metabolic syndrome. Based on our data, NFC fruit juice should be considered a healthy choice. Metabolomics assay is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS3943' rel='noopener noreferrer' target='_blank'>MTBLS3943</a>.Lipidomics assay is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4108' rel='noopener noreferrer' target='_blank'>MTBLS4108</a>.

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Bloody Mary (BM21)- Serial mixtures of vegetable juice /water and human plasma.

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