Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HILIC mode data

ABSTRACT: HILIC mode data

TISSUE(S): ['liver']

SUBMITTER: Zheng-Jiang Zhu

PROVIDER: OEX00021114 | NODE | 2022-11-26

REPOSITORIES: NODE

ACCESS DATA

Json Xml

Similar Datasets

RPLC mode data

Project description:RPLC mode data

| OEX00021115 | NODE

FloChIP's histone mark data obtained in multiplexing mode

Project description:FloChIP's histone mark data obtained in multiplexing mode

| PRJEB35683 | ENA

FloChIP's histone mark data obtained in multiplexing mode

Project description:FloChIP's histone mark data obtained in multiplexing mode

| PRJEB28149 | ENA

JPST000068 monolith HILIC-HeLa [Reanalysis: JPST000068]

Project description:A meter-scale monolithic silica capillary column modified with urea-functional groups for hydrophilic interaction liquid chromatography (HILIC) was developed for highly efficient separation of biological compounds. We prepared a ureidopropylsilylated monolithic silica capillary column with a minimum plate height of 12 Î¼m for nucleosides and a permeability of 2.1 Ã— 10âˆ’13 m2, which is comparable with the parameters of monolithic silica-C18 capillary columns. Over 300,000 theoretical plates were experimentally obtained in HILIC with a 4 m long column at 8 MPa; this is the best result yet reported for HILIC. A 2 m long ureidopropylsilylated monolithic silica capillary column was utilized to develop a HILIC mode LC-MS system for proteomics applications. Using tryptic peptides from human HeLa cell lysate proteins, we identified the comparable numbers of peptides and proteins in HILIC with those in reversed-phase liquid chromatography (RPLC) using a C18-modified monolithic silica column when shallow gradients were applied. In addition, approximately 5-fold increase in the peak response on average was observed in HILIC for commonly identified tryptic peptides due to the high acetonitrile concentration in the HILIC mobile phase. Since HILIC mode LC-MS shows orthogonal selectivity to RPLC mode LC-MS, it is useful as a complementary tool to increase proteome coverage in proteomics studies. [Original project description]

2023-04-01 | RPXD034323 | JPOST Repository

FloChIP's decreasing cell number data obtained in multiplexing mode

Project description:FloChIP's decreasing cell number data obtained in multiplexing mode

| PRJEB28205 | ENA

HILIC

Project description:HILIC

| OEX00026396 | NODE

Ketogulonigenium vulgare cells: co-culture mode vs. mono-culture mode

Project description:Ketogulonigenium vulgare cells: co-culture mode vs. mono-culture mode

| PRJNA162307 | ENA

NIST 1950 - HILIC Lipidomics in negative ion mode

Project description:Lipids in the reference material NIST 1950 (50 uL) were extracted accodring to the Matyash protocol. The sample was analysed in 5 technical replicates by ESI(-)-HILIC-TIMS-MS with PASEF enabled with 100 ms and 500 ms.

2022-11-30 | MSV000090817 | MassIVE

FloChIP's histone mark data obtained in multiplexing mode

Project description:By using FloChIP’s “sample multiplex” mode, we ChIPed in parallel 5 histone marks (H3K27ac, H3K4me3, H3K4me1 and H3K9me3), going from chromatin to sequencing-ready libraries, in just one day.

2019-12-08 | E-MTAB-8551 | biostudies-arrayexpress

FloChIP's histone mark data obtained in multiplexing mode

Project description:By using FloChIP’s “sample multiplex” mode, we ChIPed in parallel 5 histone marks (H3K27ac, H3K4me3, H3K27me3, H3K4me1 and H3K9me3), going from chromatin to sequencing-ready libraries, in just one day.

2020-06-26 | E-MTAB-7122 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data