Project description:Transcriptional profiling of Arabidopsis thaliana wild type (WT) comparing mechanical damage (MD) and Myzus persicae feeding (Myz). The differences in the biochemical responses to insect feeding seen when compared to the control sample prompted us to search for less obvious differences between the treatments using gene expression profiling. Biological replicates: 4 biological replicates

2012-09-18 | GSE40924 | GEO

Barley transcriptome analyses upon interaction with different aphid species identifies thionins contributing to resistance

Project description:Aphids are phloem-feeding insects that cause yield loss on a wide range of crops, including cereals such as barley. While most aphid species are limited to one or few host species, some are able to reproduce on many plants belonging to different families. Interestingly, aphid probing-behaviour can be observed on both host and non-host species indicating that interactions take place at the molecular level that may impact host range. Here, we aimed to gain insight into the interaction of barley with aphid species differing in their ability to infest this crop by analysing transcriptional responses. First, we determined colonization efficiency, settlement, and probing behaviour for the aphid species Rhopalosiphum padi, Myzus persicae and Myzus cerasi, which defined host, poor-host and non-host interactions, respectively. Analyses of barley transcriptional responses revealed gene sets differentially regulated upon the different barley-aphid interactions and showed that the poor-host interaction with M. persicae resulted in the strongest deregulation of genes. Interestingly, we identified several thionin genes strongly up-regulated upon interaction with M. persicae, and to a lesser extend upon R. padi interaction. Ectopic expression of two of these genes in Nicotiana benthamiana reduced host susceptibility to M. persicae, indicating thionins contribute to defences against aphids.

2017-01-23 | E-MTAB-5133 | biostudies-arrayexpress

Rhagoletis cerasi

Project description:Rhagoletis cerasi Raw sequence reads

| PRJNA561951 | ENA

Pseudomonas cerasi

Project description:Pseudomonas cerasi genome sequencing and annotation

| PRJEB12896 | ENA

Mitochondrial COI and morphological evidence for host specificity of the black cherry aphids Myzus cerasi (Fabricius, 1775) collected from different cherry tree species in Europe (Hemiptera, Aphididae).

Project description:Partial sequences of the mitochondrial COI gene of forty eight European and two Turkish population samples of Myzus cerasi from different winter hosts (Prunus spp.) were subjected to phylogenetic analyses. The analysed M. cerasi samples emerged as paraphyletic relative to a Myzus borealis sample used as an out-group, and formed two major clades in neighbor joining, maximum parsimony, maximum likelihood and Bayesian inference trees, corresponding to subspecies living specifically on Prunus avium and P. cerasus. Multivariate discriminant analysis (method of canonical variates) was applied to find out if morphological variation of samples correlated with mitochondrial COI and host plant information. Mean scores on the first two canonical variables clustered samples fully in accordance with their COI haplotypes and host plants confirming the existence of two morphologically similar winter host - specific subspecies of M. cerasi in Europe. No single morphological character enabled satisfactory discrimination between apterous viviparous females of the two subspecies. A three-character linear discriminant function enabled 92.37% correct identification of apterous viviparous females of M. cerasi cerasi (n = 118) and 93.64% of M. cerasi pruniavium (n = 110). A key for the morphological identification of the two subspecies is presented and their taxonomic status is discussed.

| S-EPMC3978913 | biostudies-literature

Arabidopsis thaliana wild type mechanical damage (MD) vs Myzus persicae wounding (Myz)

2012-09-18 | E-GEOD-40924 | biostudies-arrayexpress

Myzus persicae

Project description:Myzus persicae overview

| PRJNA45891 | ENA