Project description:To investigate the effect of stroke on the transcriptome of intestinal epithelial cells, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours
Project description:To investigate the effect of stroke on the trasnscriptome of intestinal muscularis macrophages, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours
Project description:To investigate the effect of stroke on the trasnscriptome of intestinal lamina propria macrophages, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours
Project description:Intraocular pressure was elevated through episkleral vein occlusion by thermic cauterization of SD rats. Animals were further sacrificed after different periods of elevated IOP and the retinal proteins were investigated for alterations regarding the relative protein level in this experimental model of glaucoma.
Project description:Tear samples collected from patients with central retinal vein occlusion (n=58) and group of healthy volunteers (n=30) were analyzed using a proteomic label-free absolute quantitative ap-proach. We found a large proportion (458 proteins with a frequency > 0.6) of tear proteome shared between healthy donors and subjects with the central retinal vein occlusion (CRVO). Comparative proteomic analysis revealed 29 proteins (p<0.05) significantly differed in their quantitative property between CRVO patients and the control group. Among them S100A6 (log (2)FC = 1.11, p<0.001), S100A8 (log(2)FC = 2.45, p<0.001), and S100A9 (log2(FC) = 2.08, p<0.001) are the most abundantly represented upregulated proteins, and β2-microglobulin was the most downregulated protein (log2(FC) = -2.13, p<0.001). Mesothelin (MSLN) was found as a protein with a significant increase (log2(FC) = 0.82, p<0.001) in the CRVO group. The selected up- and downregulated pro-teins were assembled to customize map of the CRVO-related critical protein interactions with quantitative properties. The customized map (FDR<0.01) emerged inflammation, impairment of retinal hemostasis, and immune response as the main set of processes associated with CRVO is-chemic condition. The semantic analysis encouraged the prevalence of core biological processes encompassing dysregulation of mitochondrial organization and regulation of improperly or utilization of topologically incorrect folded proteins as a consequence of oxidative stress and in-flammation escalating the ischemic condition caused by the local retinal hemostasis dysregulation. The most significantly different proteins (S100A6, S100A8, S100A9, MSLN, and β2-microglobulin) were applied for the ROC plotting and their AUC varied from 0.772 to 0.952 suggesting association with the CRVO.
Project description:profiling gene transcription in a mouse model of permanent focal cerebral ischemia that was induced by middle cerebral artery occlusion (MCAO)
Project description:To reveal the alterations of mRNA profile in cerebral ischemia-reperfusion injury in rat. The SD rats were used to established the middle cerebral artery occlusion and reperfusion (MCAO/R) model. RNA-seq were performed to identify differences in gene expression.
Project description:Intraocular pressure was elevated through episkleral vein occlusion by thermic cauterization of SD rats. Animals were further sacrificed after different periods of elevated IOP and the retinal proteins were investigated for alterations regarding the relative protein level in this experimental model of glaucoma.
Project description:The gastric barrier plays a major role in the maintanance of the distal intestinal microbiome composition. It has been shown before that the use of gastric acid suppression medication, such as proton pump inhibitors, are associated with distinctive alterations of the intestinal microbiome. Foremost, the invasion of predominantly oral bacteria, like Veillonella and Streptococcus species, were a resurring finding in previous reports.
Gastric cancer treatment includes the total or subtotal resection of the stomach which can influence the gastric acid production. However, the influence by alterations in gastric milieu after this treatment on the composition of the intestinal microbiome is not well studied.
Therefore, the intestinal microbiome of patients after total or subtotal gastrectomy and its influence on intestinal inflammation and gut permeability will be studied.
