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Drosophila melanogaster

ABSTRACT: Genome-wide maps of Yan chromatin association

PROVIDER: PRJNA148085 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR385747.fastq.gz	Fastqsanger.gz
	SRR385748.fastq.gz	Fastqsanger.gz
	SRR385749.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster

Project description:Genome-Wide Yan Chromatin Occupancy in Drosophila Embryos

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Genome-Wide Yan Chromatin Occupancy in Drosophila Embryos

Project description:The ETS transcriptional repressor Yan functions as part of a developmental switch that in response to receptor tyrosine kinase signaling, transitions from a high-Yan to a low-Yan state to direct specification of a variety of cell fates. To date very few direct target genes have been identified, nor is it clear how their expression is buffered against developmental noise to prevent inappropriate oscillations between states. Following investigation of its genome-wide chromatin occupancy profile, we noticed a striking signature at developmentally important genes whereby Yan associates with chromatin in regions of high-peak density that span multiple kilobases which partially relies upon SAM-domain mediated self-association. We speculate that the high-density Yan occupancy signature may reveal a novel mechanism that buffers the expression of critical developmental regulators against intrinsic and environmental noise. The supplementary bed file contains Yan binding regions.

2013-03-01 | GSE34038 | GEO

Genome-Wide Yan Chromatin Occupancy in Drosophila Embryos

2013-03-01 | E-GEOD-34038 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

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Drosophila melanogaster

Project description:Genome-wide maps of KDM5 binding in drosophila adults

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Drosophila melanogaster

Project description:Genome-wide maps of PARP-1-bound nucleosomes.

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Drosophila melanogaster

Project description:Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

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Drosophila melanogaster

Project description:Generation of chromatin maps for ASH1 and FSH applying ChIP-seq

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Drosophila melanogaster

Project description:Genome-wide maps of HMGD1 and H1-bound nucleosomes.

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Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

Project description:Trithorax group (TrxG) proteins counteract Polycomb silencing by an as yet uncharacterized mechanism. A well-known member of the TrxG is the histone methyltransferase Absent, Small, or Homeotic discs 1 (ASH1). In Drosophila ASH1 is needed for the maintenance of Hox gene expression throughout development, which is tightly coupled to preservation of cell identity. In order to understand the molecular function of ASH1 in this process, we performed affinity purification of tandem-tagged ASH1 followed by mass spectrometry (AP-MS) and identified FSH, another member of the TrxG as interaction partner. Here we provide genome-wide chromatin maps of both proteins based on ChIP-seq. Our Dataset comprises of 4 ChIP-seq samples using chromatin from S2 cells which was immunoprecipitated, using antibodies against Ash1, FSH-L and FSH-SL.

2013-06-30 | E-GEOD-30820 | biostudies-arrayexpress

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