Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA148081 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The ETS transcriptional repressor Yan functions as part of a developmental switch that in response to receptor tyrosine kinase signaling, transitions from a high-Yan to a low-Yan state to direct specification of a variety of cell fates. To date very few direct target genes have been identified, nor is it clear how their expression is buffered against developmental noise to prevent inappropriate oscillations between states. Following investigation of its genome-wide chromatin occupancy profile, we noticed a striking signature at developmentally important genes whereby Yan associates with chromatin in regions of high-peak density that span multiple kilobases which partially relies upon SAM-domain mediated self-association. We speculate that the high-density Yan occupancy signature may reveal a novel mechanism that buffers the expression of critical developmental regulators against intrinsic and environmental noise. The supplementary bed file contains Yan binding regions. Yan ChIP from staged Drosophila embryos
2013-03-01 | E-GEOD-34038 | biostudies-arrayexpress
Project description:Genome-wide maps of Yan chromatin association
| PRJNA148085 | ENA
Project description:The ETS transcriptional repressor Yan functions as part of a developmental switch that in response to receptor tyrosine kinase signaling, transitions from a high-Yan to a low-Yan state to direct specification of a variety of cell fates. To date very few direct target genes have been identified, nor is it clear how their expression is buffered against developmental noise to prevent inappropriate oscillations between states. Following investigation of its genome-wide chromatin occupancy profile, we noticed a striking signature at developmentally important genes whereby Yan associates with chromatin in regions of high-peak density that span multiple kilobases which partially relies upon SAM-domain mediated self-association. We speculate that the high-density Yan occupancy signature may reveal a novel mechanism that buffers the expression of critical developmental regulators against intrinsic and environmental noise. The supplementary bed file contains Yan binding regions.
2013-03-01 | GSE34038 | GEO
Project description:High-resolution genome-wide binding of Yan used to confirm the presence of high-density regions seen in ChIP-chip ChIP-Seq of Yan protein in stage 11 Drosophila embryos
2013-03-01 | E-GEOD-34040 | biostudies-arrayexpress
Project description:Nucleus is a highly structured organelle and contains many functional compartments. While the structural basis for this complex spatial organization of compartments is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat). Experimental evidence over the past decades indicates that most of the nuclear functions are at least transiently associated with the NuMat although the components of NuMat itself are poorly known. Here, we report NuMat proteome analysis from Drosophila melanogaster embryos and discuss its links with nuclear architecture and functions. In the NuMat proteome, we find structural proteins, chaperones related, DNA/RNA binding, chromatin remodeling and transcription factors. This complexity of NuMat proteome is an indicator of its structural and functional significance. Comparison of the 2D profile of NuMat proteome from different developmental stages of Drosophila embryos shows that less than half of the NuMat proteome is constant and rest of the proteins are stage specific dynamic components. This NuMat dynamics suggests a possible functional link between NuMat and the embryonic development. Finally, we also show that a subset of NuMat proteins remain associated with the mitotic chromosomes implicating their role in mitosis and possibly the epigenetic cellular memory. NuMat proteome analysis provides tools and opens up ways to understand nuclear organization and function.
2011-05-05 | PRD000255 | Pride
Project description:Genome-wide localization of Trf2 and TBP in Drosophila embryos
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Project description:Genome-wide profiling of PRC1 and PRC2 Polycomb chromatin binding in Drosophila
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Project description:wounded versus nonwounded drosophila embryos
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Project description:Genome-wide Tinman binding sites in early and late Drosophila embryos
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