Project description:We tested the general utility of XLE in a variety of human biological samples by analyzing human lung and thyroid tissues and stool samples. We quantified 32 environmental chemicals in 11 human lungs, with HCB, PCB-28 and PCB-18 being most frequently detected (10 out of 11). The commonly detected chemicals in human plasma were detected less frequently in the lung. For the 11 lungs, p,p’-DDE was detected in eight, PCB-153 in five, PBDE-47 and PCB-138 in four and PCB-180 in three. Although the plasma samples were from non-diseased individuals and the lungs were both diseased and non-diseased individuals, HCA results suggest that environmental chemical profiles in human lung may be very different from plasma.
Project description:Decellularized ECM (dECM) hydrogels derived from human lungs can provide valuable insight into patient-specific and lung pathophysiology. Herein, we present the approach and some initial results that highlight the unique characteristics of this dECM hydrogel platform and its benefits for a variety of potential future studies and applications.
