Project description:We report the application of single-molecule-based sequencing technology (XR-seq) for high-throughput profiling of nucleotide excision repair in Drosophila four developmental stages and S2 cells. By obtaining over six billion bases of sequence from UV and cisplatin damage antibodies immunoprecipitated excision DNA, we generated genome-wide nucleotide excision repair maps of Drosophila Embryo, Larva, Pupa , two gender of adults and S2 cells . We find that Drosophila performs transcription-coupled repair (TCR) at all its developmental stages. S2 cell carry out TCR response to both UV and Cisplatin damage. Finally, we show that XPC repair factor is required for both global and transcription-coupled repair in Drosophila. This study provides the mechanism of nucleotide excision repair of Drosophila in vivo and vitro response to UV and Cisplatin damage.

2022-02-25 | GSE184926 | GEO

Drosophila melanogaster

Project description:Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

| PRJNA380782 | ENA

Drosophila melanogaster

Project description:Single Feature Polymorphism (SFP) Data from Drosophila Genomic DNA

| PRJNA193149 | ENA