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Schistosoma japonicum

ABSTRACT: MicroRNAs are involved in the regulation of ovary development in the pathogenic blood fluke Schistosoma japonicum

PROVIDER: PRJNA301073 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2927290.fastq.gz	Fastqsanger.gz
	SRR2927291.fastq.gz	Fastqsanger.gz
	SRR2927292.fastq.gz	Fastqsanger.gz
	SRR2927293.fastq.gz	Fastqsanger.gz
	SRR2927294.fastq.gz	Fastqsanger.gz

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Schistosoma japonicum

Project description:MicroRNAs are involved in the regulation of ovary development in the pathogenic blood fluke Schistosoma japonicum

| PRJNA301073 | ENA

Comparative genomic hybridization of Schistosoma japonicum Chinese and Philippine isolates

Project description:Chinese and Philippine strains of the blood fluke Schistosoma japonicum present clear and distinctive phenotypes in areas of fecundity, pathology, drug sensitivity and immunology. Despite these differences large scale sequencing efforts have focused solely on Chinese mainland strain of the parasite. We have undertaken a comparative genomic hybridisation (CGH) approach to highlight some of the structural differences in the genome of two of the major geographical isolates of S. japonicum. We identified seven distinct regions of the S. japonicum genome that present differential CGH between Chinese and Philippine strains of the blood fluke Schistosoma japonicum, representing either deletion or duplication regions in the Philippine strain. Within these regions, genes that may be related to phenotypical differences are identified and discussed.

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Project description:The life cycle of schistosomes is complex, being characterised by a series of distinct parasitic and free-living phases involving an invertebrate snail host, water and a mammalian host. A custom designed oligonucleotide microarray was utilized to profile developmental gene expression in the Asian blood fluke, Schistosoma japonicum during these parasitic and free-living transitions. Total RNAs were isolated from lung schistosomula, juvenile females and males (paired but little or no egg production), adult males and females (paired with full scale egg production), eggs, miracidia, sporocysts and cercariae. We focused on the three distinct environmental phases of the lifecycle - aquatic/snail (eggs, miracidia, sporocysts, cercariae), juvenile (lung schistosomula and paired pre-egg laying adults) and adult (paired males and females, both examined separately) stages.

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