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Mus musculus

ABSTRACT: A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

PROVIDER: PRJNA360829 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5164440_1.fastq.gz	Fastqsanger.gz
	SRR5164440_2.fastq.gz	Fastqsanger.gz
	SRR5164441_1.fastq.gz	Fastqsanger.gz
	SRR5164441_2.fastq.gz	Fastqsanger.gz
	SRR5164442_1.fastq.gz	Fastqsanger.gz

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Mus musculus

Project description:A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

| PRJNA355987 | ENA

PhosphoRiboTrap sequencing data of chemogenetic MCH neuron activation

Project description:We report phosphoRiboTrap experiments result which aim to explore the nature of the cell types in the Median Eminence (ME) regulated as a consequene of chemogenetic MCH neuron activation. Control and MCH-hM3Dq mice were 12 hrs-fasted and i.p. injected with CNO (3 mg/kg), Arcuate (ARC) and median eminence expat were extracted for preciptation of S6-marked ribosomes from both groups of mice. Extracted RNA from Immunoprecipitated ribosomes (IP) and total tissue (ARC+ME) from each mouse were subjected to deep mRNA sequencing. By analyzing the overlap of genes enriched in the IP/Input of MCH neuron activated mice with previously identified cell types using single cell mRNA sequencing of cells in the mediobasal hypothalamus (Campbell et al., 2017), we identified gene clusters which were activated upon MCH neuron activation.

2020-05-07 | GSE146019 | GEO

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence

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Irx3 and Irx5 regulate hypothalamic postnatal neurogenesis and leptin sensitivity (DROP-seq)

Project description:Drop-Seq single cell RNA sequencing of hypothalamic arcuate nucleus and median eminence from wildtype and Irx3/5 double heterozygous mice

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A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence. Please see below link for searchable cluster-based gene expression.

2017-01-31 | GSE93374 | GEO

Irx3 and Irx5 regulate hypothalamic postnatal neurogenesis and leptin sensitivity (10X Genomics scRNA-seq)

Project description:Single cell sequencing of Ins2-Cre;ptdTomato+ FACS sorted cells from micro-dissected hypothalamic arcuate nucleus and median eminence of wildtype and Irx3/5 double heterozygous mice.

2021-02-18 | GSE154969 | GEO

Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

Project description:The median eminence (ME) of the hypothalamus is a structure that rapidly adapts to nutrient availability. We have used single-cell RNA sequencing to characterize the transcriptional profiles of cells specifically in the median eminence in the fasted and refed states. In our study, we focus on characterizing the oligodendrocyte population of the ME, and identifying how subpopulations respond to nutrient availability.

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Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities

Project description:Here we aim to decipher the actions of Irx5 in the regulation of obesity and metabolic abnormalities. We employed a mouse model homozygous for an Irx5-knockout (Irx5KO) allele and conducted droplet based single-cell RNA sequencing (Drop-seq) in the hypothalamic arcuate-median eminence (ARC-ME), which is the main control center for sensing and integrating feeding regulatory signals.

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3'-UTR-seq of Mus musculus female and male hypothalamus across the pubertal transition

Project description:The hypothalamus is a functionally and cellularly complex tissue controlling many developmental processes, including puberty. While key hormonal aspects of puberty regulation in the hypothalamus are well established, understanding the genes, cell-types, and epigenetic mechanisms underlying and regulating puberty is limited. Here, we performed 3’-UTR-seq on the hypothalamus from both sexes of C57BL/6J mice at 5 ages spanning pubertal transition (postnatal days 12, 22, 27, 32, 37) (4-5 replicates per sex at each age) to examine genome-wide age- and sex-biased trends in gene expression in a cell-type aware manner. Sample collection, RNA extraction, and sequencing was completed using the same protocols and on the same mice as PMID: 3622112 (E-MTAB-9459). QuantSeq 3’mRNA-seq libraries were constructed from total RNA using an automated method with Agilent NGS Workstation. The resulting single-end libraries were sequenced at SickKids TCAG core on the Illumina v4 flow cell with SR50 bp cycles extended to 68 bp. A customized pipeline was developed and used for the analysis of reads obtained (PMID: 36221127 for details). Processed reads were mapped to mouse genome (mm10).

2023-04-01 | E-MTAB-12340 | biostudies-arrayexpress

Bidirectional Introgression between Mus musculus domesticus and Mus spretus.

Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.

| S-EPMC8784167 | biostudies-literature

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