Project description:T cells are the main responding arm of the immune system to allografts. One mechanism that may be encouraging tolerance to allografts is T regulatory cells, a CD4+ T cell phenotype that displays antigen-directed immune suppression. T regulatory cells are reduced after allografting in the graft draining lymph node compared to the syngeneic graft draining lymph node, and miRNAs may be responsible for this decrease in suppressive cells. We used microarrays to detail what miRNAs are dysregulated after allografting in purified CD4+ T cells to identify what miRNAs are hindering the expansion of pro-tolerogenic T regulatory cells.
Project description:Extracellular adenosine triphosphate (eATP) is a signaling molecule that affects T cell function via the ionotropic P2X7 receptor. The study of effector/memory T cells isolated from mice with deletion of P2rx7, the gene encoding for P2X7, allowed understanding the impact of P2X7 activity on T cell function in the eATP-rich tumor microenvironment. To explore the the transcriptional impact of the lack of P2rx7 in CD4+ naïve and TEM cells, we performed genome-wide expression profiling of ex vivo purified CD4+ naïve and TEM cells from WT and P2rx7-/- mice
Project description:In this study, we compared the proteomes of mouse CD4+Foxp3+ regulatory T cells (Treg) and CD4+Foxp3- conventional T cells (Tconv) in order to build a data set of proteins differentially regulated in these two cell populations. The data set contains mass spectrometry results from the analysis of 7 biological replicates of Treg/Tconv cell samples purified by flow cytometry, each experiment performed from a pool of 4-5 mice. Global proteomic analysis of each sample was performed by single-run nanoLC-MS/MS, using chromatographic separation of peptides on 50cm C18 reverse-phase columns, with either a 480min gradient on LTQ-Velos orbitrap mass spectrometer (replicates 1 and 2) or a 300min gradient on Q-Exactive orbitrap mass spectrometer (replicates 3-7). Several MS injection replicates were performed for some experiments, leading to 27 raw files composing the data set. The detailed description of each analysis (file name, sample type, biological replicate number, MS technical replicate number, MS instrument used, sample name in MaxQuant ouput) is given in the table “Files list.txt”.
Project description:We recently showed that the Slamf6 protein isoform Slamf6-H1 markedly diminishes T cell–dependent disease upon introduction of a Slamf6-H1–expressing transgene into the lupus-prone Sle1b mouse, which lacks this isoform. In this study we purified CD4+ T cells from Sle1b, Sle1b.Slamf6-H1, or B6 mice and a global gene expression profile was analyzed.
Project description:Identifying BTLA interacting proteins in mouse CD4+ effector T cells expressing BTLA at endogenous levels and after stimulation with pervanadate.