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Metabolically active community of anammox biomass under different nitrogen strength

ABSTRACT: Metabolically active community of anammox biomass under different nitrogen strength Raw sequence reads

PROVIDER: PRJNA432607 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6664660_1.fastq.gz	Fastqsanger.gz
	SRR6664660_2.fastq.gz	Fastqsanger.gz
	SRR6664661_1.fastq.gz	Fastqsanger.gz
	SRR6664661_2.fastq.gz	Fastqsanger.gz
	SRR6664662_1.fastq.gz	Fastqsanger.gz

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Metabolically active community of anammox biomass under different nitrogen strength

Project description:Metabolically active community of anammox biomass under different nitrogen strength Raw sequence reads

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Effects of Fe(II) on anammox community activity and functional genes expression

Project description:Four Fe(II) concentrations (0.03, 0.09, 0.12 & 0.75 mM) were tested to investigate the stimulation and inhibition effects of ferrous iron on anammox bacterial activity. RNAs were extracted from the cultures, and the synthesized cDNAs by reverse transcription were used to carry out GeoChip analysis, by which the functional communities and expression level differences in functional genes under different Fe(II) concentrations conditions were obtained, and the response of anammox bacteria to Fe(II) stimulation and inhibition are speculated.

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Project description:The physiology of the planctomycetal anammox bacteria makes them particularly special because they share features with all three domains of life. Anammox bacteria have been reported recently to produce surface-layer proteins, which represent the outermost layer and provide structure, shape and protection under extreme conditions. Furthermore, we report on the unique cell surface-layer glycosylation of the anammox bacterium Ca. Kuenenia stuttgartiensis as revealed by a newly established glycoproteomics approach. This approach enables untargeted exploration of prokaryotic protein glycosylation from (high-resolution) shotgun proteomics data directly.

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Evaluating the suitability of granular anammox biomass for nitrogen removal from vegetable tannery wastewater.

Project description:In the present study, the potential inhibitory effect of biologically pre-treated vegetable tannery wastewater (TW) on anammox granular biomass was evaluated. Beside high organic and chemicals load, vegetable TW are characterised by high salinity and high tannins concentration, the latter belonging to a group of bio-refractory organic compounds, potentially inhibitory for several bacterial species. Recalcitrant tannin-related organic matters and salinity were selected as the two potential inhibitory factors and studied either for their separate and combined effect. Parallel batch tests were performed, with biomass acclimated and non-acclimated to salinity, testing three different conditions: non-saline control test with non-acclimated biomass (CT); saline control test with acclimated biomass (SCT); vegetable tannery wastewater test with acclimated biomass (TWT). Compared with non-saline CT, the specific anammox activity in tests SCT and TWT showed a reduction of 28 and 14%, respectively, suggesting that salinity, at conductivity values of 10 mS/cm (at 25 °C), was the main impacting parameter. As a general conclusion, the study reveals that there is no technical limitation for the application of the anammox process to vegetable TW, but preliminary biomass acclimation as well as regular biomass activity monitoring is recommended in case of long-term applications. To the best of our knowledge, this is the first work assessing the impact of vegetable TW on anammox biomass.

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