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Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

ABSTRACT: Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

PROVIDER: PRJNA495799 | ENA |

REPOSITORIES: ENA

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Selective eradication of leukemia cells with reprogramming factors

Project description:Selective eradication of leukemia cells with reprogramming factors

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Selective eradication of leukemia cells with reprogramming factors

Project description:This SuperSeries is composed of the SubSeries listed below.

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Targeting of apoptosis gene loci by reprogramming factors leads to selective eradication of leukemia cells.

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Project description:Recent reports have proposed a new paradigm for obtaining mature somatic cell types from fibroblasts without going through a pluripotent state, by briefly expressing canonical iPSC reprogramming factors Oct4, Sox2, Klf4 and c-Myc (abbreviated as OSKM), in cells expanded in lineage differentiation promoting conditions. Here we apply genetic lineage tracing for endogenous Nanog, Oct4 and X chromosome reactivation during OSKM induced trans-differentiation, as these molecular events mark final stages for acquisition of induced pluripotency. Remarkably, the vast majority of reprogrammed cardiomyocytes or neural stem cells derived from mouse fibroblasts via OSKM mediated trans-differentiation were attained after transient acquisition of pluripotency, and followed by rapid differentiation. Our findings underscore a molecular and functional coupling between inducing pluripotency and obtaining “trans-differentiated” somatic cells via OSKM induction, and have implications on defining molecular trajectories assumed during different cell reprogramming methods. poly RNA-Seq and Chromatin accesibility (ATAC-seq) were measured during conversion of mouse embryonic fibroblasts to neural stem cells using OSKM trans-differentiation method, as well as in mouse emrbyonic fibroblasts, iPSCs and mouse ESCs.

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Selective elimination of leukemia cells by reprogramming factors

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