Project description:We used RNA-seq to evaluate the global programme of gene expression after Dox inducible Yamanaka factors ectopic expression during this biological process.

2019-09-23 | GSE121122 | GEO

Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

Project description:We used ATAC-seq to evaluate the global programme of chromtain accessibility after Dox inducible Yamanaka factors ectopic expression during this biological process.

2019-09-23 | GSE121121 | GEO

Selective eradication of leukemia cells with reprogramming factors

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-09-21 | GSE121123 | GEO

Selective elimination of leukemia cells by reprogramming factors

Project description:To investigate the molecular mechanism for the elimination of leukemia cells after Dox induced OSKM expression, we sorted MLL-AF9 leukemia cells from Dox untreated and treated groups (48h and 72h) leukemic mice for gene expression profiling analysis.

2019-09-23 | GSE64414 | GEO

Targeting of apoptosis gene loci by reprogramming factors leads to selective eradication of leukemia cells.

Project description: Not available

| S-EPMC6898631 | biostudies-literature

OSKM factors cooperatively engage chromatin to initiate reprogramming

Project description:Reprogramming cells from one fate to another, using transcription factors, generates cells for research and potential therapy, yet little is known about the initial engagement of reprogramming factors with the genome. We mapped the interactions between Oct4, Sox2, Klf4, and c-Myc (OSKM) and the human genome during the first 48 hours of cellular reprogramming to pluripotency. Unlike that reported in ES/iPS cells, we find extensive overlap in the initial binding of OSKM, demonstrating that the initial regulatory network differs markedly from that in pluripotency. OSK act as pioneer factors for c-Myc, and c-Myc enhances the engagement of OSK, including at many genes that are required for conversion to pluripotency. Distal enhancer sites in closed chromatin dominate the initial OSKM distribution. Hierarchical chromatin binding during reprogramming resembles that employed during development. Four chIP-seq data sets (Oct4, Sox2, Klf4, and c-Myc) are included, one lane per factor, no replicates. Also included is an input lane from the same conditions and two mock lentiviral controls (no exogenous OSKM factors) treated with Oct4 IP and c-Myc IP.

2012-11-16 | E-GEOD-36570 | biostudies-arrayexpress

Human transcription factors responsive to initial reprogramming are predominantly legitimate during iPSC reprogramming

Project description:Human transcription factors responsive to initial reprogramming are predominantly legitimate during iPSC reprogramming

| PRJNA668803 | ENA