Ontology highlight
ABSTRACT:
PROVIDER: PRJNA552228 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Background: The possible impact of changes in diet composition for the intestinal microbiome is mostly studied after some days of adaptation to the diet of interest. The question arises if few days are enough to reflect the microbial response to the diet by changing the community composition and function. The present study investigated the fecal microbiome of pigs in a time span of four weeks after a dietary change to get an insight of the needed adaptation period. Four different diets were used differing in either protein source (field peas meal vs. soybean meal) or the concentration of calcium and phosphorus (CaP). Results: Twelve pigs were sampled at seven time points within four weeks after the dietary change. Fecal samples were used to sequence the 16S rDNA amplicons, to analyse the microbial proteins via LC-MS/MS and to determine the SCFA production. The analysis of OTU abundances and quantification values of proteins showed a significant separation of three periods of time (p=0.001). Samples from the first day are used to define the ‘Zero phase’, samples of weeks one and two are combined as ‘metabolic phase’ and an ‘equilibrium phase’ was defined based on samples from week three and four. Only in this last phase, a separation according to the supplementation of CaP was significantly detectable (p=0.001). No changes were found based on the corn-soybean meal or corn-field peas administration. The analysis of possible factors causing this significant separation showed only an overall change of bacterial members and functional properties. The metaproteomic approach yields a total of about 9700 proteins, which were used to deduce possible metabolic functions of the bacterialcommunity.
2017-10-26 | PXD006224 | Pride
Project description:The purpose of this study is to explore the effect of adding Lithospermum purpurea to the diet of soybean meal instead of 50% fish meal on the intestinal health of pearl gentian Grouper through Transcriptome
2023-06-27 | GSE235735 | GEO
Project description:This study was designed to address key questions concerning the use of alternative protein sources for animal feeds and addresses aspects such as their nutrient composition and impact on gut function. We used casein (CAS), spray dried porcine plasma (SDPP), soybean meal (SBM), and yellow meal worm (YMW) as protein sources. We have investigated the use of intestinal organoids as a model to test the effects of different protein sources on the intestinal epithelium. Mouse enteroids were exposed to different undigested protein sources (4% w/v, viz. soybean meal, SBM; casein, CAS; spray dried plasma protein, SDPP; and yellow meal worm, YMW) or DMEM as a control. Microarrays were used to detail the global gene expression.
2018-06-19 | GSE98051 | GEO
Project description:In this study we conducted a randomized, controlled, cross-over single-meal study
comparing a meal with highly fermented yogurt and cheese, and a meal with beef
and pork meatballs. Postprandial urine samples from 17 subjects were collected
sequentially after each meal up to 24 hr and analyzed by untargeted metabolomics
through an UHPLC-qTOF.
| MSV000095739 | MassIVE
Project description:The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn (Zea mays) in the US. Annual crop rotation between corn and soybean (Glycine max) disrupts the corn-dependent WCR lifecycle and was widely adopted to manage WCR. However, this strategy selected for a rotation-resistant (RR) variant with reduced ovipositional fidelity to cornfields. Previous studies indicated that RR-WCR adults exhibit greater tolerance of soybean tissue diet, different gut physiology, and host-microbe interactions compared to wild-types (WT). To identify genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA-sequencing analyses on RNA libraries from different WCR phenotypes (RR and WT) fed with corn or soybean diets. Differential gene expression analyses and network-based methods were used to identify gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses on these modules were then conducted to understand their potential functions and biological importance.
2015-11-23 | GSE62480 | GEO