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Quantitative analysis of AP-1 dependent gene expression in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

ABSTRACT: Quantitative analysis of AP-1 dependent gene expression in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

PROVIDER: PRJNA580366 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10363221_1.fastq.gz	Fastqsanger.gz
	SRR10363221_2.fastq.gz	Fastqsanger.gz
	SRR10363222_1.fastq.gz	Fastqsanger.gz
	SRR10363222_2.fastq.gz	Fastqsanger.gz
	SRR10363223_1.fastq.gz	Fastqsanger.gz

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Quantitative analysis of AP-1 dependent gene expression in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

Project description:Down-regulation of genes belonging to the JUN, FOS, and ATF families of transcription factors has previously been described. In this study, we demonstrate that the loss of AP-1 subunit gene expression leads to a decrease in AP-1 enhancer occupancy. CRISPR/Cas9 mediated depletion of AP-1 subunits demonstrates that loss of AP-1 subunit gene expression results in a perturbation of extracellular matrix-associated gene expression. This work establishes AP-1 as an important transcription factor in uterine leiomyoma gene transcription.

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Genome-wide map of H3K27Ac chromatin state in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

Project description:Genome-wide map of H3K27Ac chromatin state in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

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Genome-wide map of H3K27Ac chromatin state in CRISPR/Cas9 negative control and AP-1 depleted human uterine smooth muscle cells (HUtSMCs).

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Transcription profiling of human Gene Expression in Uterine Smooth Muscle Tumors (including normal myometrium, benign uterine leiomyoma, malignant uterine and extra-uterine leiomyosarcoma)

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Project description:Comparison of Gene Expression in Uterine Smooth Muscle Tumors (including normal myometrium, benign uterine leiomyoma, malignant uterine and extra-uterine leiomyosarcoma. Total RNA was isolated with Trizol and converted to labeled cRNA by standard Affymetrix protocols. Before analysis, Avg Diff values were normalized to a sum of 3 million for each probe set on the chip and values less than 20 were adjusted to 20 to allow log scaling in subsequent analysis. Keywords: other

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