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Single cell reconstruction of human basal cell diversity in normal and IPF lung (single-cell RNAseq)

ABSTRACT: Single cell reconstruction of human basal cell diversity in normal and IPF lung (single-cell RNAseq)

PROVIDER: PRJNA601420 | ENA |

REPOSITORIES: ENA

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Single cell reconstruction of human basal cell diversity in normal and IPF lung (single-cell RNAseq)

Project description:Declining lung function in patients with interstitial lung disease is accompanied by epithelial remodeling and progressive scarring of the gas-exchange region. There is a need to better understand the contribution of basal cell hyperplasia and associated mucosecretory dysfunction to the development of idiopathic pulmonary fibrosis (IPF). Single cell RNA sequencing was used to map epithelial cell types of the normal and IPF human airway. Organoid and ALI cultures were used to investigate functional properties of basal cell subtypes. We confirmed that Notch2 maintains undifferentiated basal cells and restrict basal-to-ciliated differentiation, and present evidence that Notch3 functions to restrain secretory differentiation. When characterizing single cell transcriptomes of the IPF lung we found a bias towards accumulation of the secretory primed basal cell subset.

2020-07-09 | GSE143706 | GEO

Human basal cell diversity in normal and IPF lung (bulk RNAseq)

Project description:Declining lung function in patients with interstitial lung disease is accompanied by epithelial remodeling and progressive scarring of the gas-exchange region. There is a need to better understand the contribution of basal cell hyperplasia and associated mucosecretory dysfunction to the development of idiopathic pulmonary fibrosis (IPF).We confirmed that Notch2 maintains undifferentiated basal cells and restrict basal-to-ciliated differentiation, and present evidence that Notch3 functions to restrain secretory differentiation. When characterizing single cell transcriptomes of the IPF lung we found a bias towards accumulation of the secretory primed basal cell subset.

2020-07-09 | GSE143705 | GEO

Human basal cell diversity in normal and IPF lung

Project description:Human basal cell diversity in normal and IPF lung

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IPF Cell Atlas

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Human basal cell diversity in normal and IPF lung (bulk RNAseq)

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IPF Cell Atlas

Project description:Single Cell RNAseq of Whole Lung Dissociates from IPF, COPD and control lungs

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Single cell RNAseq reconstruct normal and IPF lung epithelium

Project description:Single cell RNAseq reconstruct normal and IPF lung epithelium

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Single cell RNA Sequencing Identifies G-protein Coupled Receptor 87 as a Basal Cell Marker Expressed in Distal Honeycomb Cysts in Idiopathic Pulmonary Fibrosis

Project description:Idiopathic Pulmonary Fibrosis (IPF) is a devastating and life-threatening lung disease characterized by epithelial reprogramming and increased extracellular matrix deposition leading to loss of lung function. A prominent histopathological structure in the IPF lung are aberrant bronchioles filled with mucus, referred to as honeycomb cysts. These heterogeneous bronchiolized areas feature clusters of simple epithelium with Keratin (KRT)5+ basal-like cells interspersed with pseudostratified epithelium containing differentiated, hyperplastic epithelial cells as well as aberrant ciliated cells. Recent single-cell RNA sequencing studies of the lung epithelium shed further light into cellular subtypes unique to IPF, including basaloid KRT5-/KRT17+ cells present in the distal lung. However, IPF distal bronchiole cell subtypes still remain poorly characterized and their disease contribution remains underinvestigated. Using single-cell RNA sequencing of enriched EpCAM+ lung cells of the distal IPF lung, we identified G-protein coupled receptor (GPR) 87, as a marker of distal bronchioles and KRT5+ IPF basal cells contributing to impaired airway differentiation and honeycombing.

2022-05-01 | GSE190889 | GEO

Single cell RNAseq reconstruct normal and IPF lung epithelium

Project description:Tissue fibrosis is a common pathological outcome of chronic disease that markedly impairs organ function leading to morbidity and mortality. In the lung, idiopathic pulmonary fibrosis (IPF) is an insidious and fatal interstitial lung disease associated with declining pulmonary function. Single cell RNA sequencing was used to map epithelial cell types of the normal human airway and alveolaor as well as IPF explant tissue.

2020-09-24 | GSE146981 | GEO

Basal-like cell-conditioned medium exerts anti-fibrotic effects in vitro and in vivo

Project description:We performed a single-cell transcriptome analysis of basal-like cells cultured from peripheral lung tissue of patients with idiopathic pulmonary fibrosis (IPF).

2022-02-16 | GSE145439 | GEO

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