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M6A methylation regulates the fate of endogenous retrovirus transcripts [CRISPR/Cas9]

ABSTRACT: m6A methylation regulates the fate of endogenous retrovirus transcripts [CRISPR/Cas9]

PROVIDER: PRJNA607749 | ENA |

REPOSITORIES: ENA

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m6A methylation regulates the fate of endogenous retrovirus transcripts

Project description:m6A methylation regulates the fate of endogenous retrovirus transcripts

| PRJNA607745 | ENA

m6A methylation regulates the fate of endogenous retrovirus transcripts [CRISPR/Cas9]

Project description:Using a genome-scale CRISPR knockout screening in mouse embryonic stem cells, we identify m6A RNA methylation as an important regulatory component that restricts the activity of endogenous retroviruses of the IAP (intracisternal A-particles) family. The m6A methylation of IAP mRNAs occurs on their 5’ end, is catalyzed by the complex of methyltransferase-like (METTL)3/METTL14 proteins whose depletion, along with other complex subunits, namely WTAP and ZC3H13, leads to increased IAP transcript abundance. Using auxin-dependent degron, we show that rapid removal of METTL3, METTL14 and ZC3H13 further increases IAP RNA abundance in a progressive and reversible fashion. Finally, we demonstrate that m6A RNA reduces the stability of IAP transcripts

2020-04-13 | GSE145615 | GEO

m6A methylation regulates the fate of endogenous retrovirus transcripts [CUT & RUN]

Project description:m6A methylation regulates the fate of endogenous retrovirus transcripts [CUT & RUN]

| PRJNA656303 | ENA

m6A methylation regulates the fate of endogenous retrovirus transcripts [RNA-seq]

Project description:m6A methylation regulates the fate of endogenous retrovirus transcripts [RNA-seq]

| PRJNA606740 | ENA

m6A methylation regulates the fate of endogenous retrovirus transcripts [RIP-seq]

Project description:m6A methylation regulates the fate of endogenous retrovirus transcripts [RIP-seq]

| PRJNA606743 | ENA

m6A methylation regulates the fate of endogenous retrovirus transcripts [CUT & RUN]

2020-08-11 | GSE156007 | GEO

m6A methylation regulates the fate of endogenous retrovirus transcripts [RIP-seq]

2020-04-13 | GSE145315 | GEO

m6A methylation regulates the fate of endogenous retrovirus transcripts [RNA-seq]

2020-04-13 | GSE145309 | GEO

m6A methylation regulates the fate of endogenous retrovirus transcripts

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-13 | GSE145616 | GEO

m6A RNA modification controls cell fate transition in mammalian embryonic stem cells

Project description:N6-methyl-adenosine (m6A) is the most abundant modification on messenger RNAs and is linked to human diseases, but its functions in mammalian development are poorly understood. Here we reveal the evolutionary conservation and function of m6A by mapping the m6A methylome in mouse and human embryonic stem cells. Thousands of messenger and long noncoding RNAs show conserved m6A modification, including transcripts encoding core pluripotency transcription factors. m6A is enriched over 3M-bM-^@M-^Y untranslated regions at defined sequence motifs, and marks unstable transcripts, including transcripts turned over upon differentiation. Genetic inactivation or depletion of mouse and human Mettl3, one of the m6A methylases, led to m6A erasure on select target genes, prolonged Nanog expression upon differentiation, and impaired ESCM-bM-^@M-^Ys exit from self-renewal towards differentiation into several lineages in vitro and in vivo. Thus, m6A is a mark of transcriptome flexibility required for stem cells to differentiate to specific lineages. Examing m6A modification differences in two different cell types

2014-10-20 | E-GEOD-52600 | biostudies-arrayexpress

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