Project description:Ten cattle have been challenged with two Lumpy Skin Disease Virus (LSDV). They were sampled for whole blood immediately before (pre) and three and seven days after (post) infection challenge with two virus strains (H vs. O). The whole RNA-sequencing was done, and 150bp paired reads were assembled as the transcriptome. It was then computationally analyzed to find the differentially expressed genes (DGE) that enrich the gene ontology (GO) terms and KEGG pathways. Depending on the challenged LSDV strain, they influence the host response differently.
Project description:Proteomic analysis of lumpy skin disease virion (strain KSGP 0240), a poxvirus of the Capripoxvirus genus responsible of ruminant diseases in numerous countries.
Project description:Lumpy Skin Disease (LSD) is an endemic viral infection in Africa, newly emerging in Europe and becoming a global threat. There is host variation in response to infections in field studies and under carefully controlled artificial infections. It’s sometimes around 50% in clinical trials. This study aims to understand the mechanisms underlying this diversity and find the determinants to distinguish between susceptible (recovered) and resistant (non-recovered) animals through Differential Gene Expression (DGE) analysis. Five approximately 6-month-old male Holstein bulls were experimentally infected with LSDV. RNA sequencing was done for whole blood samples collected five days prior (pre-infection) to 3, 7, and 15 days after the infection (post-infection) challenge. After the challenge, two animals recovered, and three did not.
Project description:In this study, we used RNA-sequencing to compare the transcriptional programmes in cESC, elicited either by stimulation with recombinant interferon-α or by infection with the attenuated Fowlpox virus vaccine strain (FP9), the canarypox virus vaccine strain (CNPV), the Modified Vaccinia Ankara (MVA) virus or PBG98, a mild vaccine strain of infectious bursal disease virus.
2019-05-09 | GSE127203 | GEO
Project description:Genomic Sequence of Lumpy Skin Disease Virus Isolated from Taiwan
Project description:Analysis of gene expression in lungs of C57BL/6J mice that develop chronic airway disease phenotypes after a single Sendai virus infection, compared with mice treated with UV-inactivated virus. Keywords: disease state analysis Whole lung RNA was analyzed from 3 mice per condition per time point 49 days post infection, Sendai virus versus UV-inactivated virus, C57BL/6J mouse strain.