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Proteomic analysis of lumpy skin disease virion (strain KSGP 0240).

ABSTRACT: Proteomic analysis of lumpy skin disease virion (strain KSGP 0240), a poxvirus of the Capripoxvirus genus responsible of ruminant diseases in numerous countries.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine) Lumpy Skin Disease Virus

TISSUE(S): Cell Culture

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD037293 | Pride | 2024-01-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F225486.dat	Other
	F225486_p0.05.mzid.gz	Mzid
	F225487.dat	Other
	F225487_p0.05.mzid.gz	Mzid
	F225488.dat	Other

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Publications

Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomics.

Schlosser-Perrin Léo L Holzmuller Philippe P Fernandez Bernard B Miotello Guylaine G Dahmani Noureddine N Neyret Aymeric A Bertagnoli Stéphane S Armengaud Jean J Caufour Philippe P

Journal of virology 20230922 10

<h4>Importance</h4><i>Lumpy skin disease virus</i> (LSDV) is the causative agent of an economically important cattle disease which is notifiable to the World Organisation for Animal Health. Over the past decades, the disease has spread at an alarming rate throughout the African continent, the Middle East, Eastern Europe, the Russian Federation, and many Asian countries. While multiple LDSV whole genomes have made further genetic comparative analyses possible, knowledge on the protein composition ...[more]

PMID: 37737587

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Virion proteome of Cafeteria roenbergensis virus strain BV-PW1

Project description:This project describes the protein composition of the Cafeteria roenbergensis virus (CroV, strain BV-PW1: TaxID 693272) particle, a giant marine DNA virus that infects the heterotrophic nanoflagellate microeukaryote C. roenbergensis. CroV is a member of the Nucleo-Cytoplasmic Large DNA Virus clade and related to Acanthamoeba polyphaga mimivirus. CroV possesses a DNA genome of ~730 kilobase pairs that encodes 544 predicted proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins. Predicted functions could be assigned to 37% of these proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. This study shows that giant DNA virus particles contain more than one hundred viral proteins that include specific enzymatic functions.

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Proteomic analysis of a commensal Staphylococcus epidermidis strain in different pH conditions.

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An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus

Project description:The virion proteins of Kaposi Sarcoma Associated Herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined detected 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via Ultra-High Resolution Qq-Time-Of-Flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins.

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Gene expression profile after injection of recombinant deleted non-virion protein gene (NV) VHSV

Project description:The function of the non-virion protein (NV) of the Viral hemorrhagic septicemia virus (VHSV) has been long questioned but it still remains unknown. We report here the differences in gene expression profiles of trouts infected with deleted NV VHSV or wild type VHSV so that it could shed some light on the issue. Eight fingerlings of rainbow trout were used for the experiment. Four of them were injected with deleted NV VHSV and the rest were injected with wild type VHSV as a control. Head kidney and spleen of each trout were collected 2 days post-injection and total RNA was extracted.

2013-08-17 | E-GEOD-37330 | biostudies-arrayexpress

Gene expression profile after injection of recombinant deleted non-virion protein gene (NV) VHSV

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Determination of MHV virion genome poly(A) tail length and 3' end additions.

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Gene expression profile after injection of rhabdoviral recombinant protein NV [PBS vs. NV]

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Mass spectrometry-based characterization of the virion proteome and phosphoproteome of human cytomegalovirus

Project description:The assembly of human cytomegalovirus (HCMV) virions is an orchestrated process that requires, as an essential prerequisite, the complex crosstalk between viral structural proteins. So far, however, the mechanisms governing the successive steps in the constitution of virion protein complexes remained elusive. Protein phosphorylation is a key regulator determining the sequential changes in conformation, binding, dynamics and stability of proteins in the course of multiprotein assembly. Here, we present new results to complete knowledge on HCMV virion proteome and phosphoproteome. Thus, a novel dataset of viral and cellular proteins contained in HCMV virions has been generated, providing a basis for future analyses of individual phosphorylation steps and sites involved in the orchestrated assembly of HCMV virion-specific multiprotein complexes.

2020-06-05 | PXD012921 | Pride

The architecture of the SARS-CoV-2 RNA genome inside virion

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| PRJNA655427 | ENA

Profiling selective packaging of host RNA and viral RNA modification in the SARS-CoV-2 virion

Project description:Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling on host RNA packaging were mostly focused on retroviruses. Which host RNAs are packaged in other viruses at the transcriptome level has not been thoroughly examined. Here we apply both small RNA and large RNA sequencing of SARS-CoV-2 virions from six individual isolates in Vero cell cultures to profile packaging of host RNAs in a coronavirus and to explore SARS-CoV-2 genomic RNA modifications. We find selective packaging of specific tRNAs, tRNA fragments and signal recognition particle RNA into SARS-CoV-2 virions. Virions from all individual clones package the same set of host RNAs, suggesting a common mechanism of selective packaging. We estimate that a SARS-CoV-2 virion contains up to 1 SRP RNA and 4 tRNA molecules. We identify tRNA modification differences between the virion-packaged tRNAs and those in the uninfected host cells. Furthermore, we find subgenomic viral RNAs in the virions, and uncharacterized candidate modifications in the SARS-CoV-2 genomic RNA. Our results reveal an under-explored aspect of viral-host interaction that may be explored for viral therapeutics.

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