Project description:The tails of stage NF50 Xenopus tropicalis tadpoles were amputated and samples were collected at 0 and 3 days post amputation. 10 spinal cords were isolated for each time point. After cell dissociation, single cell RNAseq was performed using 10X Genomics platform
Project description:We defined genome-wide regulatory inputs of the T-box transcription factors Brachyury (Xbra), Eomesodermin (Eomes) and VegT that maintain neuro-mesodermal stem cells and determine their bipotential fates in the Xenopus tropicalis frog embryo. Binding profiles for Xbra, Eomes and VegT in X. tropicalis embryos (ChIP-Seq)
Project description:This dataset contains nano-LC-MS/MS analyzed samples of the domestic (house dust) mite Blomia tropicalis, which is a major source of allergens in tropical and subtropical regions. For proteomic analyses, four sample types, each with three biological replicates, were prepared: (i) 1000 individually collected adult mites, (ii) pools of mites of different ages, including eggs; (iii) water extracts of feces, and (iv) detergent-buffer extracts of the remaining pellet of the water extract. Laboratory culture of B. tropicalis was maintained as described previously at the Crop Research Institute, Prague, Czechia. Mites were mass reared in tissue culture flasks on a house dust mite diet (HDMd) in a large population (approximately 4 weeks) when the diet is almost consumed. This study was supported by LUAUS23082 of the Czech Ministry of Education, Youth and Sports.
Project description:We used the soil nematode Caenorhabditis elegans, a well-characterized genomics model organism, as the test organism and a species-specific, genome-wide 44K-oligo probe microarray for gene expression analysis. Our goal was to discern toxicological mechanisms for three insensitive munitions formulation IMX-101 constituents 2,4-dinitroanisole (DNAN), nitroguanidine (NQ) and nitrotriazolone (NTO) at the molecular level.
Project description:To investigate the effect of increased population density on gene expression in the nematode Caenorhabditis elegans we grew worms as solitary animals or in a ten animals population. We then performed gene expression profiling analysis using data obtained from RNA-seq of worms grown in both types of conditions (3 biological replications per condition)
2023-08-29 | GSE241786 | GEO
Project description:Anglerfish population genomics
