Proteomics

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Proteomic analysis of Blomia tropicalis bodies and feces

ABSTRACT: This dataset contains nano-LC-MS/MS analyzed samples of the domestic (house dust) mite Blomia tropicalis, which is a major source of allergens in tropical and subtropical regions. For proteomic analyses, four sample types, each with three biological replicates, were prepared: (i) 1000 individually collected adult mites, (ii) pools of mites of different ages, including eggs; (iii) water extracts of feces, and (iv) detergent-buffer extracts of the remaining pellet of the water extract. Laboratory culture of B. tropicalis was maintained as described previously at the Crop Research Institute, Prague, Czechia. Mites were mass reared in tissue culture flasks on a house dust mite diet (HDMd) in a large population (approximately 4 weeks) when the diet is almost consumed. This study was supported by LUAUS23082 of the Czech Ministry of Education, Youth and Sports.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Blomia Tropicalis (ncbitaxon:40697)

SUBMITTER: Tomas Erban  

PROVIDER: MSV000091854 | MassIVE | Thu May 04 01:26:00 BST 2023

SECONDARY ACCESSION(S): PXD041972

REPOSITORIES: MassIVE

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Predicting Blomia tropicalis allergens using a multiomics approach.

Hubert Jan J   Vrtala Susanne S   Sopko Bruno B   Dowd Scot E SE   He Qixin Q   Klimov Pavel B PB   Harant Karel K   Talacko Pavel P   Erban Tomas T  

Clinical and translational allergy 20231001 10

<h4>Background</h4>The domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite has not been sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis thus far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by the World Health Organization (WHO)  ...[more]

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