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MicroRNA profiles in KC (Kupffer cells) after partial hepatoctomy

ABSTRACT: microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

PROVIDER: PRJNA667934 | ENA |

REPOSITORIES: ENA

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mRNA and microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

Project description:mRNA and microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

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microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

Project description:The 70% resection promotes migration of Ly6C+CD11b+ monocytes/macrophages to the remnant liver accompanied by a reduction in its CD206+ macrophage content. mRNA and microRNA expression profiles were studied by NanoString technology and verified by a reverse transcription-based qPCR assay. Corresponding microtranscriptomic profiles cannot be unambiguously defined as pro- or anti-inflammatory. Their typical features include elevated expression of leukocyte chemoattractant factors, and many of the differentially expressed sequences are related to the control of cell growth and metabolic processes in the liver.

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microRNA profiles in KC (Kupffer cells) and MNCs (blood monocytes):

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mRNA and microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

Project description:This SuperSeries is composed of the SubSeries listed below.

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Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells

Project description:Self-renewing tissue-resident macrophages are thought to be exclusively derived from embryonic progenitors. However, whether circulating monocytes can also give rise to such macrophages has not been formally investigated. Here we use a new model of diphtheria toxin-mediated depletion of liver-resident Kupffer cells to generate niche availability and show that circulating monocytes engrafted in the liver, gradually adopt the transcriptional profile of their depleted counterparts and become long-lived self-renewing cells. Underlining the physiological relevance of our findings, circulating monocytes also contribute to the expanding pool of macrophages in the liver shortly after birth, when macrophage niches become available during normal organ growth. Thus, like embryonic precursors, monocytes can and do give rise to self-renewing tissue-resident macrophages if the niche is available to them. Clec4F+ Kupffer cells were isolated and sorted from livers from adult WT mice or KC-DTR or KC-DTR littermate control mice +/- 50ng DT at indicated timepoints. 19 samples (arrays) in total. RNA was isolated, amplified with Nugene pico kit, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 1.0 ST Arrays.

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Infiltrating monocyte-derived macrophages and resident kupffer cells display different ontogeny and functions in acute liver injury

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