Project description:Nanosynbacter lyticus type strain TM7x was the first cultivated member of the broadly prevelent, but poorly udnerstood Candidate Phylum Radiation super-phylum. TM7x was shown to be an obligate epibiont with a host range including Schaalia odontolyticus strain XH001. The process of infecting a naive host goes through multiple phases, from an initial binding and interaction phase we call the initial encounter, to a rapid die off of infected host cells, killing phase, followed by regrowth of the host cells, regrowth phase, and finally a stable symbiosis between the species, stable symbiosis.To obtain a better understanding of the process by which these species establish a stable symbiosis, we measured the transcriptome using RNA sequencing across the course of infection. We sampled infected host, XH001/TM7x, during the initial interaction, regrowth, and after attaining stable symbiosis, as well as naive host controls grown under the same culture conditions.
Project description:Investigation of whole genome gene expression level changes in S. pneumoniae KCTC 5080T, S. mitis KCTC 3556T, S. oralis KCTC 13048T, and S. pseudopneumoniae CCUG 49455T. This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains. A one chip study using total RNA recovered from S. pseudopneumoniae CCUG 49455T with three strain. For the the transcriptome of S. pseudopneumoniae CCUG 49455T was analyzed using the S. pneumoniae R6 microarray platform and compared with those of S. pneumoniae KCTC 5080T, S. mitis KCTC 3556T, and S. oralis KCTC 13048T strains.