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MiRNA expression in differentiating primary human bronchial epithelial cells cultured at air-liquid-interface

ABSTRACT: miRNA expression in differentiating primary human bronchial epithelial cells cultured at air-liquid-interface

PROVIDER: PRJNA690774 | ENA |

REPOSITORIES: ENA

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miRNA expression in differentiating primary human bronchial epithelial cells cultured at air-liquid-interface

Project description:We performed microarray analysis of miRNA expression in differentiating primary human bronchial epithelial cells. The goal was to identify miRNAs that are dynamically expressed under airway epithelial development. Cells were cultured at air-liquid-interface and were harvested day 4, 6, 8, 11, 13, 15, 18, 20 and 22 for analysis.

2021-01-09 | GSE164448 | GEO

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Project description:Bronchial epithelial basal cells in Air-Liquid interface culture differentiate and reconstitute a bronchial epithelium in 3-4 weeks following a tightly controlled genetic programme where specific sets of genes are up or down-regulated. We used microarrays to detail the global programme of gene expression at the on-set of differentiation in absence or presence of TGFb and identified up- and down-regulated genes during this process.

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Effect of air-liquid interface stimulation on gene expression during keratinocyte differentiation by three-dimensional culture

Project description:To investigate how air-liquid interface stimulation induces epidermal differentiation, we carried out RNA-seq on three-dimensional culture with and without air exposure.

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Strong correlation between air-liquid interface cultures and in vivo transcriptomics of nasal brush biopsy

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