LAN - FCE018
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA693281 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To examine the impact of gp130-mediated, pro-cholinergic neurokine intervention on the small RNA systems in female and male human neuronal cells, we exposed cultured LA-N-2 cells (female) to 100 ng/ml ciliary neurotrophic factor (CNTF), and LAN-5 cells (male) to 10 ng/ml CNTF. RNA sequencing of time points at 30/60 minutes and 2/4 days of CNTF exposure shows a significant response and an “immediate early”/”long term” dichotomy in differentially expressed miRNAs.
2019-10-15 | GSE132951 | GEO
Project description:RNA-seq analysis of LAN-1 neuroblastoma cells treated with G9a inhibitor BIX-01294.
| PRJEB35417 | ENA
Project description:Exposure to light at night (LAN) has been associated with serious pathologies, including obesity, diabetes and cancer. Recently we showed that 2 hours of LAN impaired glucose tolerance in rats. Several studies have suggested that the autonomic nervous system (ANS) plays an important role in communicating these acute effects of LAN to the periphery. Here, we investigated the acute effects of LAN on the liver transcriptome of male Wistar rats. Expression levels of individual genes were not markedly affected by LAN, nevertheless pathway analysis revealed clustered changes in a number of endocrine pathways. Subsequently, we used selective hepatic denervations (sympathetic (Sx), parasympathetic (Px), total (Tx, i.e., Sx plus Px), sham) to investigate the involvement of the ANS in the effects observed. Surgical removal of the sympathetic or parasympathetic hepatic branches of the ANS resulted in many, but small changes in the liver transcriptome, including a pathway involved with circadian clock regulation, but it clearly separated the four denervation groups. On the other hand, analysis of the liver metabolome was not able to separate the denervation groups, and only 6 out of 78 metabolites were significantly up- or downregulated after denervations. Finally, removal of the sympathetic and parasympathetic hepatic nerves combined with LAN exposure clearly modulated the effects of LAN on the liver transcriptome, but left most endocrine pathways unaffected. Conclusion: One-hour light-at-night acutely affects the liver transcriptome. Part of this effect is mediated via the nervous innervation, as a hepatectomy modulated and reduced the effect of LAN on liver transcripts.
2018-10-10 | GSE120978 | GEO
Project description:Exposure to light at night (LAN) has been associated with serious pathologies, including obesity, diabetes and cancer. Recently we showed that 2 hours of LAN impaired glucose tolerance in rats. Several studies have suggested that the autonomic nervous system (ANS) plays an important role in communicating these acute effects of LAN to the periphery. Here, we investigated the acute effects of LAN on the liver transcriptome of male Wistar rats. Expression levels of individual genes were not markedly affected by LAN, nevertheless pathway analysis revealed clustered changes in a number of endocrine pathways. Subsequently, we used selective hepatic denervations (sympathetic (Sx), parasympathetic (Px), total (Tx, i.e., Sx plus Px), sham) to investigate the involvement of the ANS in the effects observed. Surgical removal of the sympathetic or parasympathetic hepatic branches of the ANS resulted in many, but small changes in the liver transcriptome, including a pathway involved with circadian clock regulation, but it clearly separated the four denervation groups. On the other hand, analysis of the liver metabolome was not able to separate the denervation groups, and only 6 out of 78 metabolites were significantly up- or downregulated after denervations. Finally, removal of the sympathetic and parasympathetic hepatic nerves combined with LAN exposure clearly modulated the effects of LAN on the liver transcriptome, but left most endocrine pathways unaffected. Conclusion: One-hour light-at-night acutely affects the liver transcriptome. Part of this effect is mediated via the nervous innervation, as a hepatectomy modulated and reduced the effect of LAN on liver transcripts.
2018-10-10 | GSE120979 | GEO
Project description:8 neuroblastoma (NB) cell lines (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) their methylome is determined by sequencing after MBD2-capture using MethylCollector (ActiveMotif) 8 NB cell lines were included (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) in this study. After shearing (fragments of about 200 bp), DNA was captured using MBD2-capture (MethylCollector - ActiveMotif) followed by library preparation and multiplexing. Captured sequence tags were sequenced paired-end (2 x 45 bp) on Illumina GAIIx.
2012-09-13 | E-GEOD-31353 | biostudies-arrayexpress
Project description:high throughput sequencing of the metagenome of mexican children
2020-03-16 | GSE143207 | GEO
Project description:8 neuroblastoma (NB) cell lines (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) were profiled on the Affymetrix HGU-133plus2,0 platform before and after treatment with DAC (2'-deoxy-5-azacytidine) to investigate the influence on expression after inhibiting DNA-methylation 8 NB cell lines were included (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1), before and after treatment with 3 micromolars of DAC for 3 days
2012-09-13 | E-GEOD-31229 | biostudies-arrayexpress
Project description:The dioxaphospho-lane ring in the title compound, C(20)H(17)O(3)P, adopts an envelope conformation about one of the ring carbons. The benzene rings of the compound do not form face-to-face ?-? inter-actions, instead weak C-H?? inter-actions occur between adjacent mol-ecules. The methine H atoms on the dioxaphospho-lane ring form weak C-H?O hydrogen bonds to the oxide group of an adjacent mol-ecule.
| S-EPMC3152137 | biostudies-literature
Project description:8 neuroblastoma (NB) cell lines (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) their methylome is determined by sequencing after MBD2-capture using MethylCollector (ActiveMotif)
2012-09-13 | GSE31353 | GEO
Project description:The five-membered ring in the title compound, C(6)H(13)O(3)P, exists in an envelope conformation with one of the ring C atoms at the flap position. The coordination geometry around the P atom is a distorted tetra-hedron. The crystal structure is stabilized by several weak C-H?O and P-H?O hydrogen bonds, forming a three-dimensional network.
| S-EPMC3213595 | biostudies-literature