Project description:Anopheles arabiensis from Jinja, Uganda resistant to permethrin (1hr exposure) were compared to sympatric controls and two pyrethroid susceptible colonies of An. arabiensis (Moz and Dongola)
Project description:Complex gene flow boundaries among sympatric Anopheles vector mosquito populations revealed by genome-wide SNP genotyping.<br><br>Normalized data is provided in archive E-MTAB-338.additional.zip on the FTP site for this experiment.
Project description:MicroRNA and other types of small regulatory RNAs play a crucial role in the regulation of gene expression in eukaryotes. Several distinct classes of small regulatory RNAs have been discovered in recent years. To extend the repertoire of small regulatory RNAs characterized in mammals and identify miRNA expressed upon viral infection we used Illumina’s ultrahigh throughput sequencing approach. Results presented in this study extend our knowledge of the biology and evolution of small regulatory RNAs in mammals and illuminate mechanisms of small RNA biogenesis and function. Keywords: Transcriptome analysis
Project description:Total RNAs from exosomes was used for miRNA library preparation and sequencing.The preparation and sequencing of exosome miRNA were performed by Ribobio (Guangzhou, China). First, total RNA samples were fractionated by using 15% Tris-borate-EDTA (TBE) polyacrylamide gel (Invitrogen), then small RNAs ranging from 18 to 30 nucleotides were used for library preparation. Amplification of these small RNAs was then accomplished by PCR. Next, the Illumina HiSeq 2500 platform was used to sequence these RNAs.
