Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA-seq of skeletal muscle from young and old mice

ABSTRACT: Single cell RNA-seq of skeletal muscle from young and old mice

PROVIDER: PRJNA723271 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single cell RNA-seq of skeletal muscle from young and old mice

Project description:We report the scRNA-seq of mononuclear cells isolated from skeletal muscle of young and old mice.

2022-05-20 | GSE172410 | GEO

RNA-seq of skeletal muscle of young, old, and senolytics treated mice

Project description:We report RNA-seq of skeletal muscle (quadriceps) of young (6-month), old (24-month), and senolytic (Dasatinib + quercetin) treated mice

2022-05-20 | GSE184348 | GEO

Expression data from skeletal muscle of young, old and old calorie restricted mice

Project description:We investigated age-related changes in the transcriptional profile of skeletal muscle in 5 month old (young) and 25 month old (old) C57BL/6NHsd mice using high density oligonucleotide arrays (22,690 transcripts probed). We identified 712 transcripts that are differentially expressed in young (5 month old) and old (25-month old) mouse skeletal muscle. Caloric restriction (CR) completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P<0.05), while CR significantly lowered expression levels for these genes as compared to control fed old mice (P<0.05). Age-related induction of p53-related genes was observed in multiple tissues, but was not observed in SOD2+/- and GPX4+/- mice, suggesting that oxidative stress does not mediate the observed age-related increase in expression. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. These observations support a role for p53-mediated apoptotic activity in mammalian aging. Keywords: aging, calorie restriction, muscle, p53

2006-12-01 | GSE6323 | GEO

RNA-seq of single myofibers isolated from skeletal muscle of young and old mice

Project description:We report RNA-seq of single myofibers with different p21 expression level isolated from EDL muscle of young and old mice.

2022-05-20 | GSE172254 | GEO

RNA-seq of skeletal muscle of young, old, and senolytics treated mice

Project description:RNA-seq of skeletal muscle of young, old, and senolytics treated mice

| PRJNA764199 | ENA

Deletion of KvB2 leads to loss of muscle function in young and old mice

Project description:To investigate the skeletal muscle function and aging phenotype of KvB2 null mice we utilized the young and old KvB2 KO mice

2023-07-01 | GSE233996 | GEO

RNA-seq of single myofibers isolated from skeletal muscle of young and old mice

Project description:RNA-seq of single myofibers isolated from skeletal muscle of young and old mice

| PRJNA722527 | ENA

Comparison of Wild type and Pofut1-deleted skeletal muscle in young and old mice

Project description:Pofut1 is an essential gene that glycosylates proteins containing EGF-like repeats, including Notch Receptors (NotchRs). Work in mice and in Drosophila has shown that O-fucosylation by Pofut1 is required for NotchR ligands to bind to and activate NotchRs. As such, Pofut1 deletion in skeletal myofibers allows for an analysis of potential functions and molecular changes of Pofut1 in skeletal muscle that derive from its expression in skeletal myofibers. In this study we compared gene expression profiles between quadriceps muscles in young (2 month) and old (17 month) mice where Protein O-fucosyltransferase 1 (Pofut1) was deleted specifically in skeletal myofibers via use of a human skeletal alpha actin Cre transgene (Scre) and a loxP flanked Pofut1 gene (SCreFF) and mice which bore the only the Scre transgene but did not have floxed Pofut1 alleles (SCre++).

2017-03-27 | GSE94768 | GEO

Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging WGBS]

Project description:To uncover new pathways that are important for skeletal muscle stem cell aging, we performed multiomics profiling, including transcriptomics, DNA methylomics, proteomics, and metabolomics on quiescent muscle stem cells from young and old mice. Our goals were to discover pathways that have been overlooked by isolated profiling approaches and to gain insight into which changes are causal, compensatory, correlational, and consequential. In our work, we found that glutathione metabolism is a key pathway of muscle stem cell aging that involves a compensatory feedback loop. Follow-up experiments showed that old muscle stem cells actually form a dichotomy between glutathione-high muscle stem cells and glutathione-low muscle stem cells. RNA-Seq showed that glutathione-high old muscle stem cells are able to synthesize adequate glutathione and thus compensate adequately for oxidative stress with increased glutathione turnover, while glutathione-low old muscle stem cells have failed to compensate for oxidative stress metabolically and instead show increased inflammatory signaling.

2023-03-20 | GSE152797 | GEO

Dataset on transcriptome signature of skeletal muscle of young, adult and aged mice

Project description:Skeletal muscle aging is characterized by a progressive decline in muscle mass and function, which is referred to as sarcopenia. However, the molecular mechanisms implicated in sarcopenia remain unclear. In this dataset, we include the expression data obtained from gastrocnemius muscle of young, mature adult and old C57BL6 male mice.

2021-08-18 | GSE136266 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data