Ontology highlight
ABSTRACT:
PROVIDER: PRJNA735212 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14736778.fastq.gz | Fastqsanger.gz | |||
| SRR14736779.fastq.gz | Fastqsanger.gz | |||
| SRR14736780.fastq.gz | Fastqsanger.gz | |||
| SRR14736781.fastq.gz | Fastqsanger.gz | |||
| SRR14736782.fastq.gz | Fastqsanger.gz |
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Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed. Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.
2010-10-11 | E-GEOD-24612 | biostudies-arrayexpress
Project description:Mesenchymal stem cells (MSC) are bone-marrow derived cells, capable of multipotent differentiation into connective tissues including bone, tendon and cartilage. They are an attractive source for autologous cell-based treatments for a range of clinical diseases and injuries. MSCs have been demonstrated to possess an age-related loss of cellular functions including differentiation potential and proliferation capacity; with implications for stem cell therapies in older patients. Furthermore the reduction in differentiation potential could contribute to ageing and age-related disease. Biological aging is coupled with a progressive reduction in the regulation of cellular, tissue and organ interaction, resulting in senescence. The purpose of this study was to investigate the epigenetic, RNA and protein changes in ageing MSCs in order to understand the age-related functional and biological changes required for their applications in regenerative medicine.
2016-07-22 | MSV000079981 | MassIVE
Project description:Mesenchymal stem cells (MSC) are bone-marrow derived cells, capable of multipotent differentiation into connective tissues including bone, tendon and cartilage. They are an attractive source for autologous cell-based treatments for a range of clinical diseases and injuries. MSCs have been demonstrated to possess an age-related loss of cellular functions including differentiation potential and proliferation capacity; with implications for stem cell therapies in older patients. Furthermore the reduction in differentiation potential could contribute to ageing and age-related disease. Biological aging is coupled with a progressive reduction in the regulation of cellular, tissue and organ interaction, resulting in senescence. The purpose of this study was to investigate the epigenetic, RNA and protein changes in ageing MSCs in order to understand the age-related functional and biological changes required for their applications in regenerative medicine.
2016-02-16 | PXD001952 | Pride
Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed.
2010-10-11 | GSE24612 | GEO
Project description:We previously identified multipotent stem cells within the lamina propria of the human olfactory mucosa, located in the nasal cavity. We also demonstrated that this cell type differentiates into neural cells and improves locomotor behavior after transplantation in a rat model of Parkinsonâs disease. Yet, next to nothing is known about their specific stemness characteristics. We therefore devised a study aiming to compare olfactory lamina propria stem cells from 4 individuals to bone marrow mesenchymal stem cells from 4 age- and gendermatched individuals. Using pangenomic microarrays and immunostaining with 34 cell surface marker antibodies, we show here that olfactory stem cells are closely related to bone marrow stem cells. However, olfactory stem cells exhibit also singular traits. By means of techniques such as proliferation assay, cDNA microarrays, RT-PCR, in vitro and in vivo differentiation, we report that, when compared to bone marrow stem cells, olfactory stem cells display i) a high proliferation rate; ii) a propensity to differentiate into osseous cells and iii) a disinclination to give rise to chondrocytes and adipocytes. Since peripheral olfactory stem cells originate from a neural crest-derived tissue and, as shown here, exhibit an increased expression of neural cellrelated genes, we propose to name them olfactory ecto-mesenchymal stem cells (OE-MSC). Further studies are now required to corroborate the therapeutic potential of OE-MSCs in animal models of bone and brain diseases. Keywords: cell type comparison Expression profiles of olfactory ectomesenchymal stem cells, bone marrow MSC, periosteal cells, neural progenitors, and synovial fibroblasts were compared against each other and with different lineages of purified hematopoietic cells from bone marrow to characterise human olfactory ectomesenchymal stem cells molecularly
2010-10-08 | E-GEOD-24598 | biostudies-arrayexpress
Project description:The objective of this study was to elucidate age-related differences in gene expression profiles of rhesus monkey bone marrow-derived mesenchymal stem cells (rhMSC) obtained from fetal, infant, and adult donors relevant to their growth and other properties. Although a high degree of similarity was observed in the rhMSC gene expression profiles when comparing the three age groups, significant differences were found that strongly parallel gene expression profiles of human MSC. The potential functional relevance of differential gene expression was most apparent when comparing fetal and adult rhMSC transcript profiles. Overall, the observed gene expression profiles are consistent with a loss of rhMSC pluripotency and proliferative capacity with advancing donor age. In addition, these data highlight the importance of use of non-human primates as a model system for studying the properties of human stem cells. Experiment Overall Design: A total of eleven bone marrow-derived mesenchymal stem cell cultures were established from rhesus monkeys of diverse ages (ranging from gestation day 120 to 8 year old adults). Total RNA was harvested from these cultures and gene expression analyses were conducted using oligonucleotide microarrays. The expression profiles of a subset of genes was confirmed by qRT-PCR analysis. A detailed manuscript that details the results of our studies is currently under review.
2008-06-15 | E-GEOD-6814 | biostudies-arrayexpress
Project description:Evidence suggests that the bone marrow microenvironment (niches) support hematopoietic stem cells (HSCs). The cell-cell interaction between bone marrow mesenchymal stromal cells (BMSCs) and HSCs plays a crucial role in hematopoiesis. The aging of BMSCs lead to decreased ability to maintain hematopoiesis. We used microarray to determine the age-related change of BMSCs.
2017-05-12 | GSE78235 | GEO
Project description:Expression data from rat bone marrow mesenchymal stem cells
| PRJNA495385 | ENA
Project description:Human aging is associated with loss of function and regenerative capacity. Human bone marrow derived mesenchymal stromal cells (hMSCs) are involved in tissue regeneration, evidenced by their capacity to differentiate into several lineages and therefore are considered the gold standard for cell-based regeneration therapy. Tissue maintenance and regeneration is dependent on stem cells and declines with age and aging is thought to influence therapeutic efficacy, therefore, more insight in the process of aging of hMSCs is of high interest. We, therefore, hypothesized that hMSCs might reflect signs of aging. In order to find markers for donor age, early passage hMSCs were isolated from bone marrow of 61 donors, with ages varying from 17-84, and clinical parameters, in vitro characteristics and microarray analysis were assessed. Although clinical parameters and in vitro performance did not yield reliable markers for aging since large donor variations were present, genome-wide microarray analysis resulted in a considerable list of genes correlating with human age. By comparing the transcriptional profile of aging in human with the one from rat, we discovered follistatin as a common marker for aging in both species. The gene signature presented here could be a useful tool for drug testing to rejuvenate hMSCs or for the selection of more potent, hMSCs for cell-based therapy. We used microaray gene expression profiling to find expressed genes that correlated with the donor age of bone-marrow derived mesenchymal stromal cells (hMSCs) Human mesenchymal stem cells (hMSC) were biopsied from bone marrow from 61 different donors aging from 17 - 89 years old. The genome wide expression profiles was assessed using Affymetrix microarrays. The expression of genes was correlated with the age of the donors.
2012-07-20 | E-GEOD-39540 | biostudies-arrayexpress
Project description:The bone tissue undergoes constant turnover, which relies on skeletal stem cells (SSCs) and/or mesenchymal stem cells (MSCs) and their niches. SSCs/MSCs and their perivascular niche within the bone marrow are well characterized in long bones. As for cranial bones, besides bone marrow, the suture mesenchyme has been identified as a unique niche for SSCs/MSCs of craniofacial bones. However, a comprehensive study of the two different cranial stem cell niches at single-cell resolution is still lacking. In addition, during the progression of aging, age-associated changes in cranial stem cell niches and resident cells remain uncovered. In this study, we investigated age-related changes in cranial stem cell niches via single-cell RNA sequencing (scRNA-seq).
2023-09-11 | GSE235176 | GEO