Ontology highlight
ABSTRACT:
PROVIDER: PRJNA747886 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Cacao (Theobroma cacao) is a highly valuable crop with growing demands in the global market. However, cacao farmers often face challenges posed by black pod disease caused by Phytophthora spp. with P. palmivora as the most dominant. Regulations of various gene expression influence plant resistance to pathogens. One mechanism involves targeting mRNA of virulence genes in the invading pathogens, suppressing their infection. However, resistance also could be suppressed by plant-derived miRNAs that target their own defense genes. The objective of this study is to identify differentially expressed miRNAs in black pod resistant and susceptible Cacao varieties and to predict their targets in T. cacao and P. palmivora transcripts. In our research, 54 known miRNAs from 40 miRNA families and 67 Novel miRNAs were identified. As much as 17 miRNAs were differentially expressed in susceptible variety compared to resistant one, with 9 miRNAs were upregulated and 8 miRNAs downregulated. In T. cacao transcripts, the upregulated miRNAs were predicted to target several genes, including defense genes. The suppression of these defense genes can lead to a reduction in plant resistance against pathogen infection. While in P. palmivora transcripts, the upregulated miRNAs were predicted to target several genes, including P. palmivora effector genes and other important metabolism activities genes. In the future, limiting expression of miRNAs that target T. cacao's defense genes and applying miRNAs that target P. palmivora effector genes hold promise for enhancing cacao plant resistance against P. palmivora infection.
2024-02-14 | GSE244955 | GEO
Project description:We performed RNA-Seq technology for quantification of differential expression of cacao cell suspensions under light/dark conditions. By obtaining over 15 millions of reads per sample, we cuantified the differential gene expression in structural, regulatory and light signaling genes associated with flavonoid biosynthesis. Our results indicated that light treatments can effectively regulate flavonoid profiles, shifting catechin - epicatechin ratios, in particular as a response to switching from white to blue light. The results demonstrated that HY5, MYB12, ANR and LAR were differentially regulated under light/dark conditions and could be targeted by overexpression aiming to improve catechin synthesis in cell cultures. Additionally, co-expression analyses of late flavonoid biosynthetic genes ANS, ANR and LAR showed a larger number of shared partners in the dark compared to selective partners in white-blue treatment. In conclusion, our RNA-Seq analysis of cacao cells cultured under different light conditions provides a platform to dissect key aspects into the genetic regulatory network of flavonoids. These light-responsive candidate genes can be used further to modulate the flavonoid production in in vitro systems with value-added characteristics.
2021-05-16 | GSE114544 | GEO
Project description:Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). Keywords: Moniliophthora perniciosa, Witches Broom Disease, Pathogenesis, Cacao
2008-06-13 | GSE9627 | GEO
Project description:Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). Keywords: Moniliophthora perniciosa, Witches Broom Disease, Pathogenesis, Cacao
2008-06-13 | GSE9626 | GEO
Project description:integration of metabolomics and proteomics for understanding the molecular physiology of cacao seed development. Both the metabolomic and proteomic profiles of 4 developing stages' cacao seed were obtained from the mass spectrometry based platforms.
2016-05-09 | PXD002922 | Pride
Project description:Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). Keywords: Moniliophthora perniciosa, Witches Broom Disease, Pathogenesis, Cacao Pools from RNA of axenic cultures were amplified by RNA technique and labeled with Cyanine-3 CTP and Cyanine-5 CTP. Comparison was performed between biotrophic and necrotrophic mycelia, both grown in glycerol with cacao meristem extracts, using saprotrophic mycelia grown in glycerol as a control.
2010-06-25 | E-GEOD-9627 | biostudies-arrayexpress
Project description:Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). Keywords: Moniliophthora perniciosa, Witches Broom Disease, Pathogenesis, Cacao Pools from RNA of axenic cultures were amplified by RNA technique and labeled with Cyanine-3 CTP and Cyanine-5 CTP. Comparison was performed between biotrophic and necrotrophic mycelia, both grown in glycerol with cacao meristem extracts, using saprotrophic mycelia grown in glycerol as a control.
2010-06-26 | E-GEOD-9626 | biostudies-arrayexpress