Project description:The genomes of Bordetella pertussis strains with different passage histories were digested with a restriction endonuclease and separated by pulsed-field gel electrophoresis (PFGE). Each PFGE fragment was labeled and hybridized to a microarray to assess the genome content of the fragment.
Project description:Genomic content of Bordetella pertussis clinical isolates circulating in areas of intensive children vaccination. 13 isolates and one reference strain of Bordetella pertussis used.
Project description:Murine lung gene expression responses to primary and secondary infection with Bordetella pertussis. Data were compared to other parameters such as flow cytometry and multiplex immunoassays.
Project description:Bordetella pertussis, the causative agent of whooping cough, produces a microcapsule at its surface but its role in pertussis pathogenesis remained to be investigated.Absence of KpsT, a membrane-associated protein involved in the polysaccharide transport resulted in the down-modulation of a large number of virulence genes which correlated with strong attenuation in vivo.
