Melanopsin elevates locomotor activity during the wake state of the diurnal zebrafish
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ABSTRACT:
PROVIDER: PRJNA784979 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR17077500.fastq.gz | Fastqsanger.gz | |||
| SRR17077501.fastq.gz | Fastqsanger.gz | |||
| SRR17077502.fastq.gz | Fastqsanger.gz | |||
| SRR17077503.fastq.gz | Fastqsanger.gz | |||
| SRR17077504.fastq.gz | Fastqsanger.gz |
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Project description:Mammalian and fish pineals play a key role in adapting behaviour to the ambient light conditions through the release of melatonin. In mice, light inhibits nocturnal locomotor activity via the non-visual photoreceptor Melanopsin. In contrast to the extensively studied function of Melanopsin in the indirect regulation of the rodent pineal, its role in the intrinsically photosensitive zebrafish pineal has not been elucidated. Therefore, it is not evident if the light signalling mechanism is conserved between distant vertebrates, and how Melanopsin could affect diurnal behaviour. A double knockout of opn4.1 and opn4xb was generated in the diurnal zebrafish, which manifests attenuated locomotor activity during the wake state. Transcriptome sequencing gave insight into pathways downstream of Melanopsin, implying that sustained repression of the melatonin pathway is required to elevate locomotor activity during the wake state. Moreover, we show that light induces locomotor activity during the diurnal wake state in an intensity dependent manner. These observations suggest a common Melanopsin driven mechanism between zebrafish and mammals, while the diurnal and nocturnal chronotypes are inversely regulated downstream of melatonin.
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