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Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

ABSTRACT: Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

PROVIDER: PRJNA790759 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17276439_1.fastq.gz	Fastqsanger.gz
	SRR17276439_2.fastq.gz	Fastqsanger.gz
	SRR17276440_1.fastq.gz	Fastqsanger.gz
	SRR17276440_2.fastq.gz	Fastqsanger.gz
	SRR17276441_1.fastq.gz	Fastqsanger.gz

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Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

Project description:Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

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Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC transcriptome. Interestingly, we found that lactate supplementation activated both germline genes and cleavage embryo genes, indicating that lactate supplementation expands transcriptional network in mouse ESCs.

2022-02-01 | GSE191252 | GEO

Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC epigenome. Interestingly, we found that lactate supplementation stimulated H3K18 lactylation accumulation on a subset of genes, which in turn promoted transcriptional elongation. Our results indicate that lactate supplementation expands transcriptional network of mouse ESCs.

2022-02-01 | GSE192358 | GEO

Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

Project description:Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

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p53 regulates the Wnt signaling pathway in murine embryonic stem cells

Project description:Genome-wide analysis of gene expression changes in murine embryonic stem cells (R1E cells) treated with Ultraviolet and adriamycin Both p53 and the Wnt signaling pathways play important roles in tumorigenesis and development. However, few studies, particularly on a genome-wide scale, have linked these two pathways. Here we show that p53 directly regulates the Wnt signaling pathway in murine embryonic stem cells (mESCs) using an integrated genome-wide approach. A chromatin-immunoprecipitation-based microarray assay (ChIP-chip) reveals that the Wnt signaling pathway is significantly over-represented in p53 bound genes. Using gene expression microarray and real-time PCR, we demonstrate that the expressions of many Wnts are robustly induced by various stresses, including DNA damage and hypoxia that activate p53. Importantly, the activation of p53 is a prerequisite for the induction of Wnts. Moreover, conditional medium (CM) collected from ultraviolet (UV)-treated mESCs contains an anti-differentiation activity, which can be lowered by either the addition of Wnt signaling inhibitors into the CM or the reduction of p53 levels in UV-treated mESCs. These results suggest that stressed mESCs utilize the p53-Wnt signaling axis to signal neighboring mESCs to delay the differentiation. Together, our results uncover a novel connection between p53 and the Wnt signaling pathways in mediating cell-to-cell communication in mESCs, and provide insights into the functions of these two pathways in tumorigenesis and development.

2010-01-19 | GSE16428 | GEO

Expression data from induced pluripotent stem cells (iPSCs), mouse embryonic stem cells (mESCs), and mouse embryonic fibroblast (MEFs)

Project description:During reprogramming of mouse embryonic fibroblast, pluripotent genes are up-regulated. Once iPSCs are successfully reprogrammed, the global gene profiles of iPSCs are comparable to mouse ESC. We used microarrays to detail the global programme of gene expression in iPSCs, mESCs, MEFs.

2017-03-09 | GSE71255 | GEO

Mouse embryonic stem cells LCMS-MS

Project description:We hypothesized that uncovering interactions between Pfkp and other proteins could help resolve the mechanism whereby Pfkp regulates germ layer differentiation. Towards this, Pfkp was immunoprecipitated from mESCs and mass spectrometry analysis used to identify a number of potential Pfkp-interacting proteins

2023-02-23 | PXD038944 | Pride

Metabolomics Workbench ST000003 - GNPS - Metabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells

Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis

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DNA methylation changes in Dnmt3L-deficient mouse embryonic stem cells (mESCs)

Project description:DNA methylation changes in Dnmt3L-deficient mouse embryonic stem cells (mESCs)

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DNA methylation changes in Dnmt3L-deficient mouse embryonic stem cells (mESCs)

Project description:We used RRBS to analyze DNA methylation in mESC lines deficient for maternal Dnmt3L (Dnmt3L mKO), zygotic Dnmt3L (Dnmt3L KO), and both maternal and zygotic Dnmt3L (Dnmt3L mzKO). Compared to wild-type (WT) mESCs, Dnmt3L mKO mESCs exhibit severe loss of methylation at imprinted loci but no changes in global DNA methylation, Dnmt3L KO mESCs exhibit moderate loss of methylation at many Dnmt3a target regions but do not affect methylation at imprinted loci, and Dnmt3L mzKO mESCs exhibit combined changes of mKO and KO cells, with severe loss of methylation at imprinted loci and moderate loss of methylation at Dnmt3a target regions.

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