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Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

ABSTRACT: Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

PROVIDER: PRJNA791242 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17288228_1.fastq.gz	Fastqsanger.gz
	SRR17288228_2.fastq.gz	Fastqsanger.gz
	SRR17288229_1.fastq.gz	Fastqsanger.gz
	SRR17288229_2.fastq.gz	Fastqsanger.gz
	SRR17288230_1.fastq.gz	Fastqsanger.gz

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Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC epigenome. Interestingly, we found that lactate supplementation stimulated H3K18 lactylation accumulation on a subset of genes, which in turn promoted transcriptional elongation. Our results indicate that lactate supplementation expands transcriptional network of mouse ESCs.

2022-02-01 | GSE192358 | GEO

Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC transcriptome. Interestingly, we found that lactate supplementation activated both germline genes and cleavage embryo genes, indicating that lactate supplementation expands transcriptional network in mouse ESCs.

2022-02-01 | GSE191252 | GEO

Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

Project description:Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

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SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development

Project description:SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development

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Lactate regulates cell cycle and proliferation by remodeling the anaphase promoting complex

Project description:Lactate is abundant in rapidly dividing cells, due to the requirement for elevated glucose catabolism to support proliferation. However, it is not known whether accumulated lactate affects the proliferative state. Here, we deploy a systematic approach to determine lactate-dependent regulation of proteins across the human proteome. From this data, we elucidate a mechanism of cell cycle regulation whereby accumulated lactate activates the anaphase promoting complex (APC/C). Remodeling of APC/C in this way is caused by direct inhibition of the SUMO protease SENP1 by lactate. We discover that accumulated lactate binds and inhibits SENP1 by forming a complex with zinc in the SENP1 active site. SENP1 inhibition by lactate stabilizes SUMOylation of two residues on APC4, which are required to reorient APC/C to allow UBE2C binding and thus increase APC/C activity. This direct regulation of APC/C by lactate is required to stimulate timed degradation of cell cycle proteins, and efficient mitotic exit in proliferative mammalian cells. The above mechanism is initiated upon mitotic entry when lactate abundance reaches its apex. In this way, accumulation of lactate communicates the consequences of a nutrient replete growth phase to stimulate timed opening of APC/C, cell division, and proliferation. Conversely, we show that aberrant remodeling of APC/C by lactate occurs when lactate concentrations are chronically elevated, which is a hallmark of numerous cancers. Moreover, persistent lactate accumulation overcomes anti-mitotic pharmacology to drive mitotic slippage via the newfound mode of APC/C activation. Taken together, we define a biochemical mechanism through which lactate directly regulates protein function to control cell cycle and proliferation.

2023-02-07 | PXD036641 | Pride

Mdivi-1 treatment regulates embryo epigenome

Project description:We mouse early embryos with Mdivi-1 and exmained changes of embryo epigenome.

2023-05-05 | GSE221887 | GEO

Mdivi-1 treatment regulates mouse ESC epigenome

Project description:We treated mouse embryonic stem cells (ESCs) with Mdivi-1 and exmained changes of ESC epigenome.

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Identification of genes regulated by L-lactate and by DL-lactate in Lactobacillus plantarum

Project description:L. plantarum is known to possess an L-lactate inducible lactate racemase activity (Goffin et al. 2005. J. Bacteriol. 187:6750). In the present study, microarrays were used in order to identify all genes that are up-regulated by L-lactate, but not by a racemic mixture of D- and L-lactate. A mutant of L. plantarum NCIMB8826 deficient for NAD-dependent L-lactate activity (TF101; Ferain et al. 1994. 176:596), and thus producing no L-lactate, was grown in MRS medium at 28Â°C until mid-exponential phase (OD600nm 0.75). The culture was then divided into 3 sub-cultures. Optically pure sodium L-lactate (200 mM) was added to the first sub-culture (TF101 + L-lac 200 mM). An equimolar mixture of sodium D- and L-lactate (100 mM each) was added to the second sub-culture (TF101 + L/D-lac 200 mM). The third sub-culture was not treated (TF101; reference sample). The three sub-cultures were further incubated at 28Â°C for 1h30 (a time known to be sufficient for induction of lactate racemase activity by L-lactate). Cells were harvested by centrifugation. Microarray data were used ot identify genes that are specifically induced by L-lactate (comparison of TF101 with TF101 + L-lac 200 mM), but not by DL-lactate (comparison of TF101 with T101 + L/D-lac 200 mM). There are no biological replicates.

2013-07-01 | E-GEOD-43518 | biostudies-arrayexpress

SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [RNA-seq]

Project description:SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [RNA-seq]

| PRJNA449120 | ENA

SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [Bisulfite-seq]

Project description:SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [Bisulfite-seq]

| PRJNA449121 | ENA