ABSTRACT: In vivo emergence of Imipenem/relebactam resistance in KPC-producing Klebsiella pneumoniae mediated by increased blaKPC copy number and porins
Project description:pBIC-1a is a IncFIIk-IncFI blaKPC-2-producing plasmid. Transcriptomic analysis was performed to dive deeper into the biology of this prototypical successful plasmid. The transcriptional landscape of pBIC-1a was assessed without antibiotic, and differential analysis after imipenem exposure was performed on E. coli TOP10(pBIC-1a) whole transcriptome.
Project description:the genetic inactivation of Khk-C enhanced the survival of KPC-driven PDAC model even in absence of high fructose diet. Moreover Khk-C knock out decreased the viability of KPC organoids and cancer cells, the migratory capability of PDAC cells in vitro and the growth of KPC cells in vivo in a cell autonomous manner.
Project description:Recently, we have reported on a highly drug-resistant carbapenemase-producing isolate of Enterobacter cloacae (Nepal et al., Virulence. 2018; 9: 1377-1389). In the present study, we asked the question whether and, if so, how this isolate responds to a sub-inhibitory challenge with the antibiotic imipenem. To answer this question, we applied a SILAC proteomics approach that allowed the quantification of changes in the relative abundance of bacterial protein in response to imipenem. The results show that the investigated E. cloacae isolate mounts a highly specific response to counteract the detrimental effects of imipenem.
2021-08-10 | PXD013412 | Pride
Project description:Concomitant carriage of KPC-producing and non-KPC-producing Klebsiella pneumoniae ST512 due to in vivo lost of pKPC
Project description:Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.
2018-07-11 | PXD008019 | Pride
Project description:Ceftazidime-avibactam in combination with imipenem as salvage therapy for ST11 KPC-33-producing Klebsiella pneumoniae
