Project description:Pseudo-nitzschia multiseries (Ps-n) is a toxigenic marine diatom that produces the neurotoxin, domoic acid. We screened for candidate genes that may be involved in domoic acid production by determining changes in transcript profiles in Ps-n cultures that were in late exponential (low-domoic-acid-producing) vs. stationary (high-domoic-acid-producing) growth states. We also identified a number of candidate reference genes for future RT-qPCR studies, based on their stability in this study. Ps-n RNA was extracted from late exponential and stationary phase cultures. Experiments included one axenic culture, and two non-axenic cultures. Experiments were dye-swapped to account for differences in dye labeling and detection efficiencies: a) the axenic culture experiment included 4 technical replicate arrays (i.e., 2 dye-swapped experiments = 4 total hybridizations), and b) the non-axenic culture experiments each included 6 technical replicate arrays (i.e., 3 dye-swapped experiments = 6 total hybridizations).
Project description:Interventions: Analysis of bacteremia after ESD of the colon.
Primary outcome(s): Identification of bacteremia after ESD testing blood culture and 16SrRNA gene sequencing.
Study Design: Single arm Non-randomized
Project description:Comparison between Ustilago maydis cells grown in axenic culture and U. maydis cells from plant tumors. Keywords: developmental stages
2008-09-01 | GSE5804 | GEO
Project description:Whole genome sequencing of non-axenic chrysophytes
Project description:Chlamydia trachomatis D serovar was grown in axenic culture with G6P or G6P with glutamine. The data reveal the early transcriptonal regulation in the bacteria.
Project description:Comparison between Ustilago maydis cells grown in axenic culture and U. maydis cells from plant tumors. Keywords: developmental stages U. maydis FB1 cells were grown in axenic culture; corn plants were infected with a mixture of strains FB1 and FB2, and tumors were harvested 13 days post infection. SAmples were taken from two biological replicates each.